Enzymatically crosslinked gelatin hydrogel promotes the proliferation of adipose tissue-derived stromal cells

PeerJ

Haiyan Long¹  Yingqiang Guo²  Zhenghua Xiao²  Hong Qian²  Gang Yang³  Xiaomei Ren³  Kunlong Ma⁴ 

[1] Center of Engineering-Training, Chengdu Aeronautic Polytechnic, Chengdu, Sichuan, China;Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China;Department of Medical Information and Engineering, School of Electrical Engineering and Information, Sichuan University, Chengdu, Sichuan, China;Department of Orthopaedics, Yongchuan Hospital, Chongqing Medical University, Yongchuan, Chongqin,China;

关键词: Gelatin;    Hydrogel;    Crosslink;    Transglutaminase;    Stem cell;    Adipose;   

DOI  :  10.7717/peerj.2497

来源: DOAJ

【 摘 要 】

Gelatin hydrogel crosslinked by microbial transglutaminase (mTG) exhibits excellent performance in cell adhesion, proliferation, and differentiation. We examined the gelation time and gel strength of gelatin/mTG hydrogels in various proportions to investigate their physical properties and tested their degradation performances in vitro. Cell morphology and viability of adipose tissue-derived stromal cells (ADSCs) cultured on the 2D gel surface or in 3D hydrogel encapsulation were evaluated by immunofluorescence staining. Cell proliferation was tested via Alamar Blue assay. To investigate the hydrogel effect on cell differentiation, the cardiac-specific gene expression levelsof Nkx2.5, Myh6, Gja1, and Mef2c in encapsulated ADSCs with or without cardiac induction medium were detected by real-time RT-PCR. Cell release from the encapsulated status and cell migration in a 3D hydrogel model were assessed in vitro. Results show that the gelatin/mTG hydrogels are not cytotoxic and that their mechanical properties are adjustable. Hydrogel degradation is related to gel concentration and the resident cells. Cell growth morphology and proliferative capability in both 2D and 3D cultures were mainly affected by gel concentration. PCR result shows that hydrogel modulus together with induction medium affects the cardiac differentiation of ADSCs. The cell migration experiment and subcutaneous implantation show that the hydrogels are suitable for cell delivery.

【 授权许可】

Unknown