Comparative Study Reveals Insights of Sheepgrass (Leymus chinensis) Coping With Phosphate-Deprived Stress Condition

Frontiers in Plant Science

Jirui Gong¹  Xinhong Wu²  Weibo Ren²  Haomeng Yang³  Fang Huang³  Lianwei Peng⁴  Lingyu Li⁵  Peng Liu⁵ 

[1] College of Resources Science and Technology, Beijing Normal University, Beijing, China;Institute of Grassland Research, Chinese Academy of Agricultural Sciences, Hohhot, China;Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China;Shanghai Key Laboratory of Plant Molecular Sciences, College of Life Sciences, Shanghai Normal University, Shanghai, China;University of Chinese Academy of Sciences, Beijing, China;

关键词: L. chinensis;    Pi-deprivation;    low-Pi stress;    ATP synthase;    photosynthesis;   

DOI  :  10.3389/fpls.2019.00170

来源: DOAJ

【 摘 要 】

Sheepgrass [Leymus chinensis (Trin.) Tzvel] is a valuable forage plant highly significant to the grassland productivity of Euro-Asia steppes. Growth of above-ground tissues of L. chinensis is the major component contributing to the grass yield. Although it is generally known that this species is sensitive to ecosystem disturbance and adverse environments, detailed information of how L. chinensis coping with various nutrient deficiency especially phosphate deprivation (-Pi) is still limited. Here, we investigated impact of Pi-deprivation on shoot growth and biomass accumulation as well as photosynthetic properties of L. chinensis. Growth inhibition of Pi-deprived seedlings was most obvious and reduction of biomass accumulation and net photosynthetic rate (Pn) was 55.3 and 63.3%, respectively, compared to the control plants grown under Pi-repleted condition. Also, we compared these characters with seedlings subjected to low-Pi stress condition. Pi-deprivation caused 18.5 and 12.3% more reduction of biomass and Pn relative to low-Pi-stressed seedlings, respectively. Further analysis of in vivo chlorophyll fluorescence and thylakoid membrane protein complexes using 2D-BN/SDS-PAGE combined with immunoblot detection demonstrated that among the measured photosynthetic parameters, decrease of ATP synthase activity was most pronounced in Pi-deprived plants. Together with less extent of lipid peroxidation of the thylakoid membranes and increased ROS scavenger enzyme activities in the leaves of Pi-deprived seedlings, we suggest that the decreased activity of ATP synthase in their thylakoids is the major cause of the greater reduction of photosynthetic efficiency than that of low-Pi stressed plants, leading to the least shoot growth and biomass production in L. chinensis.

【 授权许可】

Unknown