Genome-Wide Investigation and Functional Verification of the ZIP Family Transporters in Wild Emmer Wheat

International Journal of Molecular Sciences

Bin Tu¹  Tao Zhang¹  Bihua Wu²  Bo Jiang²  Dengcai Liu²  Youliang Zheng²  Yarong Jin²  Wenyang Wang²  Jingshu He²  Yi Wang²  Yanling Hu²  Jia Liu²  Tiangang Qi²  Fangyi Gong²  Lianquan Zhang²  Lin Huang² 

[1] Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China;State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu 611130, China;

关键词: ZIP gene family;    wild emmer wheat;    yeast complementation;    transgenic verification;   

DOI  :  10.3390/ijms23052866

来源: DOAJ

【 摘 要 】

The zinc/iron-regulated transporter-like protein (ZIP) family has a crucial role in Zn homeostasis of plants. Although the ZIP genes have been systematically studied in many plant species, the significance of this family in wild emmer wheat (Triticum turgidum ssp. dicoccoides) is not yet well understood. In this study, a genome-wide investigation of ZIPs genes based on the wild emmer reference genome was conducted, and 33 TdZIP genes were identified. Protein structure analysis revealed that TdZIP proteins had 1 to 13 transmembrane (TM) domains and most of them were predicted to be located on the plasma membrane. These TdZIPs can be classified into three clades in a phylogenetic tree. They were annotated as being involved in inorganic ion transport and metabolism. Cis-acting analysis showed that several elements were involved in hormone, stresses, grain-filling, and plant development. Expression pattern analysis indicated that TdZIP genes were highly expressed in different tissues. TdZIP genes showed different expression patterns in response to Zn deficiency and that 11 genes were significantly induced in either roots or both roots and shoots of Zn-deficient plants. Yeast complementation analysis showed that TdZIP1A-3, TdZIP6B-1, TdZIP6B-2, TdZIP7A-3, and TdZIP7B-2 have the capacity to transport Zn. Overexpression of TdZIP6B-1 in rice showed increased Zn concentration in roots compared with wild-type plants. The expression levels of TdZIP6B-1 in transgenic rice were upregulated in normal Zn concentration compared to that of no Zn. This work provides a comprehensive understanding of the ZIP gene family in wild emmer wheat and paves the way for future functional analysis and genetic improvement of Zn deficiency tolerance in wheat.

【 授权许可】

Unknown