Optimization of Laccase Production, and Characterization of Lignin Degradation Products by Fusarium oxysporum JUMAD-053

Orbital: The Electronic Journal of Chemistry

Gabriela C.P.S. Souza¹  Renato P. Dorte¹  Robert F.H. Dekker¹  Julliana I. Simionato¹  Luciana Furnaleto Maia¹  Beatriz R Ribeiro¹  Juliana Feijó de Souza Daniel¹  Igor S. Shiraishi²  Geisa P.C. Evaristo³  Joseph A.M. Evaristo³  Dionísio Borsato⁴  Aneli M. Barbosa-Dekker⁴ 

[1] Federal University of Technology (UTFPR). Campus Londrina;Federal University of Technology (UTFPR);Laboratory for the support of technological development (LADETEC), Federal University of Rio de Janeiro.;State University of Londrina (UEL).;

关键词: biodegradation;    factorial design;    kraft lignin;    laccase;    mass spectrometry;   

DOI  :  10.17807/orbital.v13i5.1623

来源: DOAJ

【 摘 要 】

The objective of this work was to optimize the culture conditions for laccase production in the presence of Kraft lignin by Fusarium oxysporum JUMAD-053, and to evaluate the biodegradation products of lignin. The fungal isolate that presented highest laccase activity had its production optimized by a statistical factorial design 3³ in 15 experimental runs. F. oxysporum presented the highest constitutive laccase titer (5.37 U/mL). Statistical factorial design demonstrated a maximum laccase titer of 9.8 U/mL when assayed against ABTS under the conditions optimized: 1.125% (w/v) yeast extract, 0.5% (w/v) Kraft lignin and 10 days of cultivation. The maximum laccase titer when assayed on DMP was 8.4 U/mL, following the conditions optimized: 1.125% yeast extract, 0.25% Kraft lignin and 7 days of cultivation. The analysis of cultures led to identification of metabolites; two being aromatic: 2,6-dimethoxy benzoic acid and sesamin; also, fumonisin and long-chain fatty acids. As a result of the study, the maximum laccase activities of 9.8 and 8.4 U/mL measured from ABTS and DMP substrates, respectively. The search shows new sources of fungal laccase for obtaining new metabolites of biodegradation from Kraft lignin in culture medium.

 

DOI: http://dx.doi.org/10.17807/orbital.v13i5.1623

 

【 授权许可】

Unknown