The Effects of Chronic Administration of Cisplatin on Oxidative Stress in the Isolated Rat Heart

Serbian Journal of Experimental and Clinical Research

Sabo Tibor¹  Stojic Isidora²  Jeremic Jovana²  Nikolic Tamara²  Srejovic Ivan³  Zivkovic Vladimir³  Smigic Jelena³  Jakovljevic Vladimir³ 

[1] Department of General and Inorganic Chemistry, Faculty of Chemistry, University of Belgrade, Serbia;Department of Pharmacy, Faculty of Medical Sciences, University of Kragujevac, Serbia;Department of Physiology, Faculty of Medical Sciences, University of Kragujevac, Serbia;

关键词: cisplatin;    change of coronary perfusion pressure;    isolated rat heart;    oxidative stress;   

DOI  :  10.1515/sjecr-2017-0003

来源: DOAJ

【 摘 要 】

Taken into consideration that molecular and cellular mechanisms involved in cardiotoxicity are still not clear the aim of this study was to compare the production of oxidative stress parameters in the isolated rat heart between animals chronically treated with cisplatin and saline. The hearts of male Wistar albino rats (n = 24, 12 per group, age 8 weeks, body mass 250±50 g) were excised and perfused according to the Langendorff technique at gradually increased coronary perfusion pressures (40-120 cm H2O). We followed the production of superoxide anion radicals, hydrogen peroxide, and nitrites and also index of lipid peroxidation during the changes of coronary perfusion pressure (CPP) (from 40 to 120 cm H2O) in coronary venous effluent. Modifications CPP were performed in order to determined if oxidative stress is involved in coronary endothelium response in conditions of hypoxia (lower than 60 cm H2O) and hyperoxia (higher than 80 cm H2O).

【 授权许可】

Unknown