The First Genetic Map for a Psoraleoid Legume (Bituminaria bituminosa) Reveals Highly Conserved Synteny with Phaseoloid Legumes

Plants

Jafar S. Jabbari¹  Rust Turakulov¹  Matthew N. Nelson²  Daniel Real³  Jacob S. Stai⁴  Maria Pazos-Navarro⁵  Aneeta Pradhan⁶  Steven B. Cannon⁷ 

[1] Australian Genome Research Facility, Victorian Comprehensive Cancer Centre, Melbourne, VIC 3000, Australia;CSIRO Agriculture & Food, Floreat, WA 6014, Australia;Department of Primary Industries and Regional Development, South Perth, WA 6151, Australia;Interdepartmental Genetics and Genomics Graduate Program, Iowa State University, Ames, IA 50010 USA;School of Agriculture and Environment, The University of Western Australia, Perth, WA 6009, Australia;School of Biological Sciences, The University of Western Australia, Perth, WA 6009, Australia;USDA-ARS, Corn Insects and Crop Genetics Research Unit, Ames, IA 50011, USA;

关键词: legume genome evolution;    Phaseoleae;    Psoraleeae;    Ks analysis;    linkage mapping;    perennial forage legume;   

DOI  :  10.3390/plants9080973

来源: DOAJ

【 摘 要 】

We present the first genetic map of tedera (Bituminaria bituminosa (L.) C.H. Stirton), a drought-tolerant forage legume from the Canary Islands with useful pharmaceutical properties. It is also the first genetic map for any species in the tribe Psoraleeae (Fabaceae). The map comprises 2042 genotyping-by-sequencing (GBS) markers distributed across 10 linkage groups, consistent with the haploid chromosome count for this species (n = 10). Sequence tags from the markers were used to find homologous matches in the genome sequences of the closely related species in the Phaseoleae tribe: soybean, common bean, and cowpea. No tedera linkage groups align in their entirety to chromosomes in any of these phaseoloid species, but there are long stretches of collinearity that could be used in tedera research for gene discovery purposes using the better-resourced phaseoloid species. Using Ks analysis of a tedera transcriptome against five legume genomes provides an estimated divergence time of 17.4 million years between tedera and soybean. Genomic information and resources developed here will be invaluable for breeding tedera varieties for forage and pharmaceutical purposes.

【 授权许可】

Unknown