A Plant Proteinase Inhibitor from Enterolobium contortisiliquum Attenuates Pulmonary Mechanics, Inflammation and Remodeling Induced by Elastase in Mice

International Journal of Molecular Sciences

Yara Aparecida Lobo¹  Maria Luiza Vilela Oliva¹  Carla Máximo Prado²  Leandro Vilela Oliva³  Bruno Tadeu Martins-Oliveira³  Beatriz Mangueira Saraiva-Romanholo³  Renato Fraga Righetti³  Edna Aparecida Leick³  Rafael Almeida-Reis³  Iolanda de Fátima Lopes Calvo Tibério³  Osmar Aparecido Theodoro-Júnior³  Mílton de Arruda Martins³  Nathalia Montouro Pinheiro³ 

[1] Department of Biochemistry, Federal University of São Paulo, 09972-270 Diadema, Brazil;Department of Bioscience, Federal University of Sao Paulo, 09972-270 Diadema, Brazil;Department of Clinical Medicine, School of Medicine, University of São Paulo, 01246-903 São Paulo, Brazil;

关键词: COPD;    emphysema;    proteinase inhibitor;    EcTI;   

DOI  :  10.3390/ijms18020403

来源: DOAJ

【 摘 要 】

Proteinase inhibitors have been associated with anti-inflammatory and antioxidant activities and may represent a potential therapeutic treatment for emphysema. Our aim was to evaluate the effects of a plant Kunitz proteinase inhibitor, Enterolobium contortisiliquum trypsin inhibitor (EcTI), on several aspects of experimental elastase-induced pulmonary inflammation in mice. C57/Bl6 mice were intratracheally administered elastase (ELA) or saline (SAL) and were treated intraperitoneally with EcTI (ELA-EcTI, SAL-EcTI) on days 1, 14 and 21. On day 28, pulmonary mechanics, exhaled nitric oxide (ENO) and number leucocytes in the bronchoalveolar lavage fluid (BALF) were evaluated. Subsequently, lung immunohistochemical staining was submitted to morphometry. EcTI treatment reduced responses of the mechanical respiratory system, number of cells in the BALF, and reduced tumor necrosis factor-α (TNF-α), matrix metalloproteinase-9 (MMP-9), matrix metalloproteinase-12 (MMP-12), tissue inhibitor of matrix metalloproteinase (TIMP-1), endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS)-positive cells and volume proportion of isoprostane, collagen and elastic fibers in the airways and alveolar walls compared with the ELA group. EcTI treatment reduced elastase induced pulmonary inflammation, remodeling, oxidative stress and mechanical alterations, suggesting that this inhibitor may be a potential therapeutic tool for chronic obstructive pulmonary disease (COPD) management.

【 授权许可】

Unknown