Biocompatible Platinum Nanoclusters Prepared Using Bitter Gourd Polysaccharide for Colorimetric Detection of Ascorbic Acid

Biomolecules

Weifeng Lin¹  Haotian Sun²  Yu Zhao³  Lu Zhang³  Longgang Wang³  Zhiwei Liu³  Mengmeng He³  Kai Liu³  Jie Hu³ 

[1] Department of Molecular Chemistry and Materials Science, Weizmann Institute of Science, Rehovot 76100, Israel;Ocean NanoTech, LLC, San Diego, CA 92126, USA;State Key Laboratory of Metastable Materials Science and Technology, Key Laboratory of Applied Chemistry, Hebei Key Laboratory of Heavy Metal Deep-Remediation in Water and Resource Reuse, Yanshan University, Qinhuangdao 066004, China;

关键词: green synthesis;    colorimetric;    bitter gourd polysaccharide;    ascorbic acid;    detection;   

DOI  :  10.3390/biom11050647

来源: DOAJ

【 摘 要 】

Ascorbic acid is an organic compound with antioxidant properties that can protect the human body from the threat of free radicals. Therefore, it is important to detect the existence and measure the concentration of ascorbic acid to regulate its content in the human body. In this work, we prepared bitter gourd polysaccharide (BGP)-stabilized platinum nanoclusters (Pt-BGP NCs) by reacting BGP with K₂PtCl₄. Pt-BGP NCs and catalyzed the decomposition of H₂O₂ to generate •OH radicals, which could oxidize TMB to form oxidized TMB (oxTMB), indicating their peroxidase-like properties. The kinetics followed the Michaelis–Menten equation. Furthermore, the colorimetric detection of ascorbic acid using Pt-BGP NCs showed high selectivity and a low detection limit of 0.191 μM. The accuracy of real sample detection using Pt-BGP NCs was as high as 98.9%. More importantly, Pt-BGP NCs had high cell biocompatibility and extremely low hemolysis rate due to the component of BGP. In summary, the prepared Pt-BGP NCs with reductive activity and good biocompatibility have good application prospects in colorimetric detection of ascorbic acid.

【 授权许可】

Unknown