Effect of Captopril on Brain Oxidative Damage inPentylenetetrazole-Induced Seizures in Mice

Pharmaceutical Sciences

Mohammad Soukhtanloo¹  Akbar Anaeigoudari²  Fereshteh Asgharzadeh³  Mahmoud Hosseini³  Rahimeh Bargi⁴  Zohreh Mohammady⁴  Farimah Beheshti⁵ 

[1] Department of Biochemistry, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.;Department of Physiology, School of Medicine, Jiroft University of Medical Sciences, Jiroft, Iran.;Division of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.;Neurogenic Inflammation Research Center, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.;Neuroscience Research Center, Torbat Heydariyeh University of Medical Sciences, Torbat Heydariyeh, Iran.;

关键词: Captopril;    Pentylenetetrazole;    Seizures;    Mice;    Oxidative Stress;   

DOI  :  10.15171/PS.2019.38

来源: DOAJ

【 摘 要 】

Background: Frequent seizure is followed by overproduction of free radicals andbrain oxidative stress. Renin angiotensin system (RAS) hassome effects on central nervous system. We designed this research to challengethe effect of captopril as an angiotensin converting enzyme (ACE) inhibitoragainst brain oxidative stress in pentylenetetrazole (PTZ) -induced seizures inmice.Methods:The groups were including (1) Control (saline); (2)PTZ (100 mg/kg, i.p.), (3-5) PTZ- captopril (Capto) that received three dosesof Capto 10, 50 and 100mg/kg 30 minbefore PTZ injection. Latency time in the onset minimal clonic seizures (MCS)and generalized tonic-clonic seizures (GTCS) were recorded. The level ofmalondialdehyde (MDA) and total thiol, as well as superoxide dismutase (SOD)and catalase (CAT) activity in the hippocampus and cortex were measured.Results:All doses of captopril postponed the onset of MCS andGTCS. Accumulation of MDA in the brain tissues of PTZ group was higher thancontrol group, while total thiol content and CAT activity were lower.Pretreatment with captopril (100 mg/kg) diminished MDA concentration comparedwith PTZ group. Captopril (50 and 100 mg/kg) also increased the level of totalthiol groups versus PTZ group. Captopril injection (50 and 100 mg/kg) elevatedthe activity of SOD and CAT in the brain tissues. In addition captopril administrationdiminished mortality rate caused by PTZ. Conclusion:Findings demonstrated that convulsions caused by PTZwere followed by oxidative stress status in the brain tissues. Pretreatmentwith captopril attenuated the effect of PTZ on brain tissue oxidative damage.

【 授权许可】

Unknown