Canonical Wnt Signaling Promotes Formation of Somatic Permeability Barrier for Proper Germ Cell Differentiation

Frontiers in Cell and Developmental Biology

Yu Cai¹  Shun-Min Yang²  Hwei-Jan Hsu³  Ting-An Chen³  Chi-Hung Lin³  Kun-Yang Lin³  Chen-Yuan Tseng³  Yu-Ting Wang³  Lichao Luo⁴ 

[1] Department of Biological Sciences, National University of Singapore, Singapore, Singapore;Faculty of Science, University of South Bohemia, České Budějovice, Czechia;Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan;Temasek Life Science Laboratory, National University of Singapore, Singapore, Singapore;

关键词: germline stem cells;    GSCs;    escort cell protrusions;    DPP;    Wg;    TKV;   

DOI  :  10.3389/fcell.2022.877047

来源: DOAJ

【 摘 要 】

Morphogen-mediated signaling is critical for proper organ development and stem cell function, and well-characterized mechanisms spatiotemporally limit the expression of ligands, receptors, and ligand-binding cell-surface glypicans. Here, we show that in the developing Drosophila ovary, canonical Wnt signaling promotes the formation of somatic escort cells (ECs) and their protrusions, which establish a physical permeability barrier to define morphogen territories for proper germ cell differentiation. The protrusions shield germ cells from Dpp and Wingless morphogens produced by the germline stem cell (GSC) niche and normally only received by GSCs. Genetic disruption of EC protrusions allows GSC progeny to also receive Dpp and Wingless, which subsequently disrupt germ cell differentiation. Our results reveal a role for canonical Wnt signaling in specifying the ovarian somatic cells necessary for germ cell differentiation. Additionally, we demonstrate the morphogen-limiting function of this physical permeability barrier, which may be a common mechanism in other organs across species.

【 授权许可】

Unknown