Frontiers in Plant Science | |
Comprehensive Profiling and Inheritance Patterns of Metabolites in Foxtail Millet | |
Fang Zhao1 Feng Wang1 Xiaolei Feng1 Dequan Wang1 Shuangdong Li1 Xiaoming Wang1 Fengcang Qiu1 Gaolei Shi1 Guangyu Fan1 Guoliang Song1 Yali Zhang1 Xiaolei Zhang1 Wenying Zhang1 Zhihai Zhao1 Wei Wei1 Xinru Li1 Qiaofeng Yang2 Jian Shi2 Ning Li2 Xuekui Dong2 | |
[1] Institute of Millet, Zhangjiakou Academy of Agricultural Science, Zhangjiakou, China;Wuhan Metware Biotechnology Co., Ltd., Wuhan, China; | |
关键词: Setaria italica; metabolic profiling; flavonoids; phenolamides; heterosis; | |
DOI : 10.3389/fpls.2018.01716 | |
来源: DOAJ |
【 摘 要 】
Metabolomics aims at determining a sample's metabolites profile and hence provides a straight functional statement of an organism's physiological condition. Here, we investigated comprehensive profiling, natural variation and species-specific accumulation of both primary and secondary metabolites in foxtail millet using LC-MS, and inheritance patterns of metabolome in millet hybrids. The application of a broad target metabolomics method facilitated the simultaneous identification and quantification of more than 300 metabolites. The metabolic analysis of these compounds, such as flavonoids, phenolamides, hydrocinnamoyl derivatives, vitamins and LPCs, revealed their developmentally controlled accumulation, and natural variation in different tissues/varieties. Species-specific accumulation of secondary metabolites was observed based on a comparative metabolic analysis between millet and rice, such as flavonoid O-rutinosides/neohesperidosides and malonylated flavonoid O-glycosides. In analyzing the metabolic variations between hybrid progenies and their parental lines, including a photothermo-sensitive genic male sterility line and five Zhangzagu varieties, metabolic overdominant, and dominant patterns of inheritance could be observed. For example, hydrocinnamoyl derivatives and feruloylated flavonoids were identified as over-parent heterosis (overdominant) metabolites in milet hybrids. Our work paves the way for developing predictors of hybrid performance and the future analysis of the biosynthesis and regulation of relevant metabolic pathways in millet.
【 授权许可】
Unknown