A Versatile Optoelectronic Tweezer System for Micro-Objects Manipulation: Transportation, Patterning, Sorting, Rotating and Storage

Micromachines

Fenghui Wang¹  Fumihito Arai²  Chunyuan Gan³  Bin Song³  Lin Feng³  Yuguo Dai³  Shuzhang Liang³  Xue Bai³  Yuqing Cao³  Chaonan Zhang³ 

[1] BEIGE Institue of Robot & Intelligent Manufacturing, Weifang 261000, China;Department of Mechanical Engineering, University of Tokyo, Tokyo 113-8656, Japan;School of Mechanical Engineering & Automation, Beihang University, Beijing 100191, China;

关键词: microparticle manipulation;    optoelectronic tweezers;    sorting;    rotating;   

DOI  :  10.3390/mi12030271

来源: DOAJ

【 摘 要 】

Non-contact manipulation technology has a wide range of applications in the manipulation and fabrication of micro/nanomaterials. However, the manipulation devices are often complex, operated only by professionals, and limited by a single manipulation function. Here, we propose a simple versatile optoelectronic tweezer (OET) system that can be easily controlled for manipulating microparticles with different sizes. In this work, we designed and established an optoelectronic tweezer manipulation system. The OET system could be used to manipulate particles with a wide range of sizes from 2 μm to 150 μm. The system could also manipulate micro-objects of different dimensions like 1D spherical polystyrene microspheres, 2D rod-shaped euglena gracilis, and 3D spiral microspirulina. Optical microscopic patterns for trapping, storing, parallel transporting, and patterning microparticles were designed for versatile manipulation. The sorting, rotation, and assembly of single particles in a given region were experimentally demonstrated. In addition, temperatures measured under different objective lenses indicate that the system does not generate excessive heat to damage bioparticles. The non-contact versatile manipulation reduces operating process and contamination. In future work, the simple optoelectronic tweezers system can be used to control non-contaminated cell interaction and micro-nano manipulation.

【 授权许可】

Unknown