Perinatal Exposure of Bisphenol A Differently Affects Dendritic Spines of Male and Female Grown-Up Adult Hippocampal Neurons

Frontiers in Neuroscience

Yasushi Hojo³  Shuji Aou⁴  Toshihiro Kominami⁵  Hinako Yoshino⁵  Suguru Kawato⁶  Mari Ogiue-Ikeda⁶  Minoru Saito⁶  Mika Soma⁶ 

[1] Bioinformatics Project, Japan Science and Technology Agency, The University of Tokyo, Tokyo, Japan;Core Research for Evolutional Science and Technology Project of Japan Science and Technology Agency, The University of Tokyo, Tokyo, Japan;Department of Biochemistry, Faculty of Medicine, Saitama Medical University, Saitama, Japan;Department of Biological Functions and Engineering, Graduate School of Life Sciences and Systems Engineering, Kyushu Institute of Technology, Wakamatsu, Japan;Department of Biophysics and Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo, Tokyo, Japan;Department of Biosciences, College of Humanities and Sciences, Nihon University, Tokyo, Japan;Department of Urology, Graduate School of Medicine, Juntendo University, Tokyo, Japan;

关键词: Bisphenol A;    perinatal exposure;    spine;    synapse;    hippocampus;    image analysis;   

DOI  :  10.3389/fnins.2021.712261

来源: DOAJ

【 摘 要 】

Perinatal exposure to Bisphenol A (BPA) at a very low dose may modulate the development of synapses of the hippocampus during growth to adulthood. Here, we demonstrate that perinatal exposure to 30 μg BPA/kg per mother’s body weight/day significantly altered the dendritic spines of the grownup rat hippocampus. The density of the spine was analyzed by imaging of Lucifer Yellow-injected CA1 glutamatergic neurons in adult hippocampal slices. In offspring 3-month male hippocampus, the total spine density was significantly decreased by BPA exposure from 2.26 spines/μm (control, no BPA exposure) to 1.96 spines/μm (BPA exposure). BPA exposure considerably changed the normal 4-day estrous cycle of offspring 3-month females, resulting in a 4∼5 day estrous cycle with 2-day estrus stages in most of the subjects. In the offspring 3-month female hippocampus, the total spine density was significantly increased by BPA exposure at estrus stage from 2.04 spines/μm (control) to 2.25 spines/μm (BPA exposure). On the other hand, the total spine density at the proestrus stage was moderately decreased from 2.33 spines/μm (control) to 2.19 spines/μm (BPA exposure). Thus, after the perinatal exposure to BPA, the total spine density in males became lower than that in females. Concerning the BPA effect on the morphology of spines, the large-head spine was significantly changed with its significant decrease in males and moderate change in females.

【 授权许可】

Unknown