Lentiviral vector transduction of spermatozoa as a tool for the study of early development

FEBS Open Bio

Carol Readhead¹  Jayesh Dudhia²  Colin Casimir³  Anil Chandrashekran⁴  Ihsan Isa⁴  Robert Winston⁴  Nicholas Dibb⁴  Adrian J. Thrasher⁵ 

[1] Biological Imaging Center, California Institute of Technology, Beckman Institute, 139-74, Pasadena, CA 91135, USA;Department of Clinical Science Services, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Herts AL9 7TA, UK;Department of Natural Sciences, School of Science & Technology, Middlesex University, The Burroughs, London NW4 4BT, UK;Department of Surgery and Cancer, Division of Cancer, Imperial College London, Hammersmith Campus, Institute of Reproductive and Developmental Biology (IRDB), Du Cane Road, London W12 0NN, UK;Molecular Immunology Unit, UCL-Institute of Child Health, 30 Guilford Street, London WC1N 1EH, UK;

关键词: Lentiviral vectors;    Spermatozoa;    Transduction;    Development;    Transgenics;    In vitro fertilisation;   

DOI  :  10.1016/j.fob.2014.02.008

来源: DOAJ

【 摘 要 】

Spermatozoa and lentiviruses are two of nature’s most efficient gene delivery vehicles. Both can be genetically modified and used independently for the generation of transgenic animals or gene transfer/therapy of inherited disorders. Here we show that mature spermatozoa can be directly transduced with various pseudotyped lentiviral vectors and used in in vitro fertilisation studies. Lentiviral vectors encoding Green Fluorescent Protein (GFP) were shown to be efficiently processed and expressed in sperm. When these transduced sperm were used in in vitro fertilisation studies, GFP expression was observed in arising blastocysts. This simple technique of directly transducing spermatozoa has potential to be a powerful tool for the study of early and pre-implantation development and could be used as a technique in transgenic development and vertical viral transmission studies.

【 授权许可】

Unknown