MiRNA-128 regulates the proliferation and neurogenesis of neural precursors by targeting PCM1 in the developing cortex

eLife

Hyunsoo Shawn Je¹  Steven George Rozen²  Eng King Tan³  Li Zeng⁴  Hidayat Lokman⁴  Paul Jong Kim⁴  Wei Zhang⁵  Zhongcan Chen⁵  Ke Zhang⁵  Lifeng Qiu⁵ 

[1] Neuroscience and Behavioral Disorders program, Duke-NUS Graduate Medical School, Singapore, Singapore;Center for Computational Biology, Duke-NUS Graduate Medical School, Singapore, Singapore;Department of Neurology, National Neuroscience Institute, Singapore, Singapore;Molecular Neurophysiology Laboratory, Signature Program in Neuroscience and Behavioral Disorders, Duke-NUS Graduate Medical School, Singapore, Singapore;Neural Stem Cell Research Lab, Research Department, National Neuroscience Institute, Singapore, Singapore;

关键词: miR-128;    neuronal precursor cells;    neocortex;    pericentriolar material 1;    neurogenesis;   

DOI  :  10.7554/eLife.11324

来源: DOAJ

【 摘 要 】

During the development, tight regulation of the expansion of neural progenitor cells (NPCs) and their differentiation into neurons is crucial for normal cortical formation and function. In this study, we demonstrate that microRNA (miR)-128 regulates the proliferation and differentiation of NPCs by repressing pericentriolar material 1 (PCM1). Specifically, overexpression of miR-128 reduced NPC proliferation but promoted NPC differentiation into neurons both in vivo and in vitro. In contrast, the reduction of endogenous miR-128 elicited the opposite effects. Overexpression of miR-128 suppressed the translation of PCM1, and knockdown of endogenous PCM1 phenocopied the observed effects of miR-128 overexpression. Furthermore, concomitant overexpression of PCM1 and miR-128 in NPCs rescued the phenotype associated with miR-128 overexpression, enhancing neurogenesis but inhibiting proliferation, both in vitro and in utero. Taken together, these results demonstrate a novel mechanism by which miR-128 regulates the proliferation and differentiation of NPCs in the developing neocortex.

【 授权许可】

Unknown