Molecular markers for Nassella trichotoma (Poaceae) to study genetic variation in New Zealand

Applications in Plant Sciences

Graeme W. Bourdôt¹  Shona L. Lamoureaux¹  Gary J. Houliston²  Ana Podolyan²  Claudia Lange²  Dagmar F. Goeke³ 

[1] AgResearch 1365 Springs Road Lincoln 7674 New Zealand;Manaaki Whenua Landcare Research 54 Gerald Street Lincoln 7608 New Zealand;Scion, 49 Sala Street Rotorua 3010 New Zealand;

关键词: Nassella tenuissima;    Nassella trichotoma;    nassella tussock;    New Zealand;    Poaceae;    simple sequence repeat (SSR) markers;   

DOI  :  10.1002/aps3.1181

来源: DOAJ

【 摘 要 】

Premise of The Study Simple sequence repeat (SSR) markers were developed for the study of genetic diversity of New Zealand Nassella trichotoma (Poaceae) and to support future studies in its native range. Methods and Results Genomic DNA was extracted from N. trichotoma leaf material and subjected to Roche 454 sequencing. From a total of 745 putative SSRs, 48 with di‐ to pentanucleotide repeats were screened, 32 primer pairs were designed, and 15 polymorphic markers were optimized for multiplex PCR on 105 N. trichotoma samples from four New Zealand regions. Each locus resulted in two to six alleles per locus, and four of the loci cross‐amplified in N. tenuissima. The mean observed and expected heterozygosity ranged from 0.00 to 0.90 and 0.00 to 0.50 per locus, respectively. Conclusions The novel SSR markers are valuable for the study of genetic diversity of N. trichotoma and might also be useful for closely related species.

【 授权许可】

Unknown