Exploring Insulin Production Following Alveolar Islet Transplantation (AIT)

International Journal of Molecular Sciences

Wojciech Kielan¹  Piotr Frelkiewicz²  Sven Stieglitz³  Tanja Khosrawipour⁴  Hien Lau⁵  Michael Alexander⁵  Jonathan Robert Todd Lakey⁵  Veria Khosrawipour⁵  Shiri Li⁶  Maya Karine Labbé⁷ 

[1] 2nd Department of General Surgery and Surgical Oncology, Wroclaw Medical University, 50-556 Wroclaw, Poland;Center for Experimental Diagnostics and Biomedical Innovations, Wroclaw University of Environmental and Life Sciences, 50-375 Wroclaw, Poland;Department Pulmonary Medicine, Petrus-Hospital Wuppertal, University of Witten-Herdecke, D-42283 Wuppertal, Germany;Department of Surgery (A), University-Hospital Düsseldorf, Heinrich-Heine University, Moorenstrasse 5, D-40225 Duesseldorf, Germany;Department of Surgery, University of California, Irvine (UCI), Orange, CA 92868, USA;Department of Surgery, Weill Medical College of Cornell University, New York, NY 10065, USA;School of Dentistry, Wroclaw Medical University, 50-367 Wroclaw, Poland;

关键词: islet;    insulin;    aerosol;    lung;    drug delivery;    transplantation;   

DOI  :  10.3390/ijms221910185

来源: DOAJ

【 摘 要 】

Recent studies have demonstrated the feasibility of islet implantation into the alveoli. However, until today, there are no data on islet behavior and morphology at their transplant site. This study is the first to investigate islet distribution as well insulin production at the implant site. Using an ex vivo postmortem swine model, porcine pancreatic islets were isolated and aerosolized into the lung using an endoscopic spray-catheter. Lung tissue was explanted and bronchial airways were surgically isolated and connected to a perfusor. Correct implantation was confirmed via histology. The purpose of using this new lung perfusion model was to measure static as well as dynamic insulin excretions following glucose stimulation. Alveolar islet implantation was confirmed after aerosolization. Over 82% of islets were correctly implanted into the intra-alveolar space. The medium contact area to the alveolar surface was estimated at 60 +/− 3% of the total islet surface. The new constructed lung perfusion model was technically feasible. Following static glucose stimulation, insulin secretion was detected, and dynamic glucose stimulation revealed a biphasic insulin secretion capacity during perfusion. Our data indicate that islets secrete insulin following implantation into the alveoli and display an adapted response to dynamic changes in glucose. These preliminary results are encouraging and mark a first step toward endoscopically assisted islet implantation in the lung.

【 授权许可】

Unknown