Unique Epigenetic Programming Distinguishes Regenerative Spermatogonial Stem Cells in the Developing Mouse Testis

iScience

Jon M. Oatley¹  Christopher B. Geyer²  Benjamin J. Hale²  Kazadi Mutoji³  Brian P. Hermann³  Ching-Hsun Eric Cheng³  I-Chung Chen³  John R. McCarrey³  Keren Cheng³ 

[1] Center for Reproductive Biology, School of Molecular Biosciences, College of Veterinary Medicine, Washington State University, Pullman, WA, USA;Department of Anatomy and Cell Biology at the Brody School of Medicine and East Carolina Diabetes and Obesity Institute, East Carolina University, Greenville, NC, USA;Department of Biology, University of Texas at San Antonio, San Antonio, TX, USA;

关键词: Developmental Genetics;    Omics;   

DOI  :  

来源: DOAJ

【 摘 要 】

Summary: Spermatogonial stem cells (SSCs) both self-renew and give rise to progenitors that initiate spermatogenic differentiation in the mammalian testis. Questions remain regarding the extent to which the SSC and progenitor states are functionally distinct. Here we provide the first multiparametric integrative analysis of mammalian germ cell epigenomes comparable with that done for >100 somatic cell types by the ENCODE Project. Differentially expressed genes distinguishing SSC- and progenitor-enriched spermatogonia showed distinct histone modification patterns, particularly for H3K27ac and H3K27me3. Motif analysis predicted transcription factors that may regulate spermatogonial subtype-specific fate, and immunohistochemistry and gene-specific chromatin immunoprecipitation analyses confirmed subtype-specific differences in target gene binding of a subset of these factors. Taken together, these results show that SSCs and progenitors display distinct epigenetic profiling consistent with these spermatogonial subtypes being differentially programmed to either self-renew and maintain regenerative capacity as SSCs or lose regenerative capacity and initiate lineage commitment as progenitors.

【 授权许可】

Unknown