Striatal adenosine A2A receptor neurons control active-period sleep via parvalbumin neurons in external globus pallidus

eLife

Hui Dong¹  Alban de Kerchove d'Exaerde²  Lu Wang²  Zhi-Li Huang²  Su-Rong Yang²  Michael Lazarus²  Rui-Xi Li³  Xiang-Shan Yuan³  Wei-Min Qu³  Serge N Schiffmann³  Yoan Cherasse³ 

[1] Department of Anatomy, Histology and Embryology, School of Basic Medical Science, Fudan University, Shanghai, China;State Key Laboratory of Medical Neurobiology, Institutes of Brain Science and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai, China;Department of Pharmacology, School of Basic Medical Science, Fudan University, Shanghai, China;

关键词: striatum;    A2A receptor;    sleep;    chemogenetics;    optogenetics;   

DOI  :  10.7554/eLife.29055

来源: DOAJ

【 摘 要 】

Dysfunction of the striatum is frequently associated with sleep disturbances. However, its role in sleep-wake regulation has been paid little attention even though the striatum densely expresses adenosine A2A receptors (A2ARs), which are essential for adenosine-induced sleep. Here we showed that chemogenetic activation of A2AR neurons in specific subregions of the striatum induced a remarkable increase in non-rapid eye movement (NREM) sleep. Anatomical mapping and immunoelectron microscopy revealed that striatal A2AR neurons innervated the external globus pallidus (GPe) in a topographically organized manner and preferentially formed inhibitory synapses with GPe parvalbumin (PV) neurons. Moreover, lesions of GPe PV neurons abolished the sleep-promoting effect of striatal A2AR neurons. In addition, chemogenetic inhibition of striatal A2AR neurons led to a significant decrease of NREM sleep at active period, but not inactive period of mice. These findings reveal a prominent contribution of striatal A2AR neuron/GPe PV neuron circuit in sleep control.

【 授权许可】

Unknown