Untargeted Metabolomics and Polyamine Profiling in Serum before and after Surgery in Colorectal Cancer Patients

Metabolites

JiWon Lee¹  Justin Jeon²  NamKyu Kim³  Ki-Yong An⁴  Jongki Hong⁵  BongChul Chung⁶  YuRa Lee⁶ 

[1] Department of Family Medicine, Yonsei University College of Medicine, Seoul 06273, Korea;Department of Sport Industry, Yonsei University, Seoul 03722, Korea;Department of Surgery, Yonsei University College of Medicine, Seoul 03722, Korea;Faculty of Kinesiology, Sport and Recreation, University of Alberta, Edmonton, AB T6G 2R3, Canada;KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Seoul 02447, Korea;Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul 02792, Korea;

关键词: colorectal cancer;    liquid chromatography-mass spectrometry;    polyamine;    tumorectomy;    metabolomics;    biomarker;   

DOI  :  10.3390/metabo10120487

来源: DOAJ

【 摘 要 】

Colorectal cancer is one of the most prevalent cancers in Korea and globally. In this study, we aimed to characterize the differential serum metabolomic profiles between pre-operative and post-operative patients with colorectal cancer. To investigate the significant metabolites and metabolic pathways associated with colorectal cancer, we analyzed serum samples from 68 patients (aged 20–71, mean 57.57 years). Untargeted and targeted metabolomics profiling in patients with colorectal cancer were performed using liquid chromatography-mass spectrometry. Untargeted analysis identified differences in sphingolipid metabolism, steroid biosynthesis, and arginine and proline metabolism in pre- and post-operative patients with colorectal cancer. We then performed quantitative target profiling of polyamines, synthesized from arginine and proline metabolism, to identify potential polyamines that may serve as effective biomarkers for colorectal cancer. Results indicate a significantly reduced serum concentration of putrescine in post-operative patients compared to pre-operative patients. Our metabolomics approach provided insights into the physiological alterations in patients with colorectal cancer after surgery.

【 授权许可】

Unknown