Establishing reference intervals for von Willebrand factor multimers

Journal of Medical Biochemistry

van Dievoet Marie-Astrid¹  Vasse Marc²  Szanto Timea³  Pikta Marika⁴  Lejniece Sandra⁵  Bautista Hector⁶  Nouadje George⁶  Smock Kristi J.⁷  Moser Karen A.⁷  Banys Valdas⁸ 

[1] Cliniques Universitaires Saint-Luc, Laboratory Department, Bruxelles, Belgium;Foch Hospital, Department of Biology & UMR INSERM 1176, Suresnes, France;Helsinki University Hospital Comprehensive Cancer Center and University of Helsinki, Department of Hematology, Coagulation Disorders Unit, Helsinki, Finland;North Estonia Medical Centre, Department of Laboratory Medicine, Tallinn, Estonia;Riga Stradins University, Riga, Latvia;Sebia, Research and Developments Department, Lisses - Evry Cedex, France;University of Utah, School of Medicine, Department of Pathology, Salt Lake City, UT, USA;Vilnius University, Faculty of Medicine, Institute of Biomedical Sciences, Department of Physiology, Biochemistry, Microbiology and Laboratory Medicine, Vilnius, Lithuania;

关键词: von willebrand factor;    von willebrand factor multimers;    quantitative analysis;    reference intervals;   

DOI  :  10.5937/jomb0-31941

来源: DOAJ

【 摘 要 】

Background: von Willebrand factor (VWF) multimers (VWF:MM) methodologies are technically difficult, laborious, time consuming, non-standardized and results vary between laboratories. A new semi automated VWF:MM assay is available for routine use (Sebia). Due to lack of reference values for VWF:MM fractions, results interpretation can be challenging in some cases. The aim of this study was to determine reference intervals for low molecular weight (LMWM), intermediate molecular weight (IMWM) and high molecular weight (HMWM) multimers. Methods: By the international cooperation initiated between 4 countries (Estonia, Latvia, France, and USA) 131 samples of relatively healthy individuals were analyzed for VWF:MM (in total 51 males and 80 non-pregnant females aged 17-69 years). Reference intervals were calculated according to CLSI C28-A3 standard. Results: The proposed reference intervals for VWF:MM were calculated for LMWM 10.4-22.5%, IMWM 22.6-37.6%, HMWM 45.6-66.6%. Age related differences were seen in IMWM and HMWM (p<0.001 and 0.038). There was no gender related difference observed. Geographically LMWM results of France were different from the other regions (p<0.05). Conclusions: Quantification of VWF:MM fractions, in addition to qualitative assessment of VWF:MM patterns, has the potential to aid in differential diagnosis of von Willebrand disease (VWD) subtypes. The reference values calculated in this study can be used in future research to establish clinical decision limits.

【 授权许可】

Unknown