Effect of Elicitation with (+)-Usnic Acid on Accumulation of Phenolic Acids and Flavonoids in Agitated Microshoots of Eryngium alpinum L.

Molecules

Barbara Thiem¹  Małgorzata Kikowska¹  Halina Ekiert²  Marta Klimek-Szczykutowicz²  Agnieszka Szopa²  Karolina Jafernik²  Elżbieta Studzińska-Sroka³ 

[1] Department of Pharmaceutical Botany and Plant Biotechnology, University of Medical Sciences in Poznan, 14 Św. Marii Magdaleny St., 61-861 Poznań, Poland;Department of Pharmaceutical Botany, Collegium Medicum, Jagiellonian University, 9 Medyczna St., 30-688 Kraków, Poland;Department of Pharmacognosy, University of Medical Sciences in Poznan, 4 Święcickiego St., 61-781 Poznań, Poland;

关键词: alpine eryngo;    microshoot cultures;    phenolic compounds;    elicitation;    HPLC-DAD analysis;   

DOI  :  10.3390/molecules26185532

来源: DOAJ

【 摘 要 】

The present work was aimed at studying the potential of elicitation on the accumulation of phenolic compounds in in vitro shoot cultures of Eryngium alpinum L., a protected plant from the Apiaceae family. The study examined the influence of (+)-usnic acid on the biomass growth as well as on the biosynthesis of the desired flavonoids and phenolic acids in the cultured microshoots. The phenolic compound content was determined by HPLC-DAD. The flavonoid of the highest concentration was isoquercetin, and the phenolic acids of the highest amount were rosmarinic acid, caffeic acid and 3,4-dihydroxyphenylacetic acid, both in the non-elicited and elicited biomass. Isoquercetin accumulation was efficiently increased by a longer elicitation with a lower concentration of lichenic compound (107.17 ± 4.67 mg/100 g DW) or a shorter elicitation with a higher concentration of acid (127.54 ± 11.34 and 108.37 ± 12.1 mg/100 g DW). Rosmarinic acid production generally remained high in all elicited and non-elicited microshoots. The highest content of this acid was recorded at 24 h of elicitation with 3.125 µM usnic acid (512.69 ± 4.89 mg/100 g DW). The process of elicitation with (+)-usnic acid, a well-known lichenic compound with allelopathic nature, may therefore be an effective technique of enhancing phenolic compound accumulation in alpine eryngo microshoot biomass.

【 授权许可】

Unknown