Obese Mice with Dyslipidemia Exhibit Meibomian Gland Hypertrophy and Alterations in Meibum Composition and Aqueous Tear Production

International Journal of Molecular Sciences

Susanne Brodesser¹  Madhavi Chintapalati²  Tiffany Bullock²  C.Wayne Smith²  RolandoE. Rumbaut²  Samuel Hanlon³  Rachel Redfern³  EugeneA. Osae³  AlanR. Burns³  Philipp Steven⁴ 

[1] CECAD Research Center, Lipidomics/Metabolomics Facility, University of Cologne, 50931 Cologne, Germany;Children’s Nutrition Research Center, Baylor College of Medicine, Houston, TX 77030, USA;College of Optometry, University of Houston, Houston, TX 77204, USA;Department of Ophthalmology, Division for Dry-Eye and Ocular GvHD, Medical Faculty, University of Cologne, 50937 Cologne, Germany;

关键词: obesity;    dyslipidemia;    meibum;    lipids;    meibomian gland dysfunction;    dry eye;   

DOI  :  10.3390/ijms21228772

来源: DOAJ

【 摘 要 】

Background: Dyslipidemia may be linked to meibomian gland dysfunction (MGD) and altered meibum lipid composition. The purpose was to determine if plasma and meibum cholesteryl esters (CE), triglycerides (TG), ceramides (Cer) and sphingomyelins (SM) change in a mouse model of diet-induced obesity where mice develop dyslipidemia. Methods: Male C57/BL6 mice (8/group, age = 6 wks) were fed a normal (ND; 15% kcal fat) or an obesogenic high-fat diet (HFD; 42% kcal fat) for 10 wks. Tear production was measured and meibography was performed. Body and epididymal adipose tissue (eAT) weights were determined. Nano-ESI-MS/MS and LC-ESI-MS/MS were used to detect CE, TG, Cer and SM species. Data were analyzed by principal component analysis, Pearson’s correlation and unpaired t-tests adjusted for multiple comparisons; significance set at p ≤ 0.05. Results: Compared to ND mice, HFD mice gained more weight and showed heavier eAT and dyslipidemia with higher levels of plasma CE, TG, Cer and SM. HFD mice had hypertrophic meibomian glands, increased levels of lipid species acylated by saturated fatty acids in plasma and meibum and excessive tear production. Conclusions: The majority of meibum lipid species with saturated fatty acids increased with HFD feeding with evidence of meibomian gland hypertrophy and excessive tearing. The dyslipidemia is associated with altered meibum composition, a key feature of MGD.

【 授权许可】

Unknown