AQP4 Antibody Assay Sensitivity Comparison in the Era of the 2015 Diagnostic Criteria for NMOSD

Frontiers in Neurology

The Australian and New Zealand NMO Collaboration¹  Roger A. Silvestrini²  Fabienne Brilot³  Sudarshini Ramanathan³  Michael H. Barnett³  Simon A. Broadley⁴  Wajih Bukhari⁴  John D. E. Parratt⁵  Angela Vincent⁶  Patrick Waters⁶  Mark Woodhall⁶  Christine S. Bundell⁷  Kerri Prain⁸ 

[1] ;Brain Autoimmunity Group, Kids Neuroscience Centre, Kids Research at the Children's Hospital, Westmead, NSW, Australia;Brain and Mind Centre, University of Sydney, Camperdown, NSW, Australia;Department of Neurology, Gold Coast University Hospital, Southport, QLD, Australia;Department of Neurology, Royal North Shore Hospital, Sydney, NSW, Australia;Oxford Autoimmune Neurology Group, Nuffield Department of Clinical Neurosciences, University of Oxford, Oxford, United Kingdom;PathWest Laboratory Medicine, Department of Immunology, QEII Medical Centre, Nedlands, WA, Australia;Pathology Queensland Central Laboratory, Division of Immunology, Royal Brisbane and Women's Hospital, Herston, QLD, Australia;School of Biomedical Science, Medicine, University of Western Australia, Nedlands, WA, Australia;School of Medicine, Gold Coast Campus, Griffith University, Southport, QLD, Australia;

关键词: neuromyelitis optica;    autoantibody;    aquaporin;    myelin oligodendrocyte glycoprotein;    astrocytopathy;    demyelination;   

DOI  :  10.3389/fneur.2019.01028

来源: DOAJ

【 摘 要 】

We have compared five different assays for antibodies to aquaporin-4 in 181 cases of suspected Neuromyelitis optica spectrum disorders (NMOSD) and 253 controls to assess their relative utility. As part of a clinically-based survey of NMOSD in Australia and New Zealand, cases of suspected NMOSD were referred from 23 centers. Clinical details and magnetic imaging were reviewed and used to apply the 2015 IPND diagnostic criteria. In addition, 101 age- and sex-matched patients with multiple sclerosis were referred. Other inflammatory disease (n = 49) and healthy controls (n = 103) were also recruited. Samples from all participants were tested using tissue-based indirect immunofluorescence assays and a subset were tested using four additional ELISA and cell-based assays. Antibodies to myelin oligodendrocyte glycoprotein (MOG) were also assayed. All aquaporin-4 antibody assays proved to be highly specific. Sensitivities ranged from 60 to 94%, with cell-based assays having the highest sensitivity. Antibodies to MOG were detected in 8/79 (10%) of the residual suspected cases of NMOSD. Under the 2015 IPND diagnostic criteria for NMOSD, cell-based assays for aquaporin-4 are sensitive and highly specific, performing better than tissue-based and ELISA assays. A fixed cell-based assay showed near-identical results to a live-cell based assay. Antibodies to MOG account for only a small number of suspected cases.

【 授权许可】

Unknown