Genetic Diversity Revealed by Microsatellites in Genus Carya

Forests

Warren Chatwin¹  Angelyn Hilton¹  Keith Kubenka¹  Xinwang Wang¹ 

[1] USDA ARS Pecan Breeding and Genetics, Somerville, TX 77879, USA;

关键词: pecan;    Carya;    section;    hickory;    SSR;    principal component analysis;   

DOI  :  10.3390/f13020188

来源: DOAJ

【 摘 要 】

The genus Carya consists of 17 species divided into 3 sections: Carya or the true hickories, Apocarya or the pecan hickories, and Sinocarya or the Asian hickories. Interspecific hybrids exist and have been used in pecan cultivar development. Nuclear and plastid microsatellite or SSR markers have been useful in distinguishing species, sections, and populations. They provide evidence for hybridity between species and can confirm heredity within crosses. As more sophisticated methods of genomic evaluation are cooperatively developed for use in pecan breeding and selection, the use of these methods will be supplemented and informed by the lessons provided by microsatellite markers, as interpreted across broad germplasm collections. In this study, over 400 Carya accessions from diverse diploid and tetraploid taxa and their interspecific hybrids, maintained at the USDA National Collection of Genetic Resources for Carya (NCGR-Carya), were analyzed using 14 nuclear and 3 plastid microsatellite markers. Principal coordinate analysis showed clear taxonomic classifications at multiple taxonomic levels along with patterns of interspecific hybridity. Evidence was also found for genetic differences associated with geographic distribution. The results indicate that this group of markers is useful in examining and characterizing populations and hybrids in the genus Carya and may help delineate the composition of a core collection to help characterize the NCGR-Carya repository collection for use in its pecan breeding program. The SSR fingerprints of the inventories of the USDA NCGR-Carya repository can also be used as a reference for identifying unknown pecan trees for growers.

【 授权许可】

Unknown