Characterization of Two Cryptic Plasmids Isolated in Haiti from Clinical Vibrio cholerae Non-O1/Non-O139

Frontiers in Microbiology

Ramunas Stepanauskas¹  Seon Y. Choi²  Daniela Ceccarelli³  Rita R. Colwell⁵  Geneviève Garriss⁶  Anwar Huq⁷  Mihai Pop⁸  Nur A. Hasan⁸ 

[1] Bigelow Laboratory for Ocean Sciences, East Boothbay, ME, United States;CosmosID Inc., Rockville, MD, United States;Department of Bacteriology and Epidemiology, Wageningen Bioveterinary Research, Lelystad, Netherlands;Department of Cell Biology and Molecular Genetics, Maryland Pathogen Research Institute, University of Maryland, College Park, MD, United States;Department of Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD, United States;Department of Microbiology, Cell Biology and Tumor Biology, Karolinska Institutet, Stockholm, Sweden;Maryland Institute of Applied Environmental Health, University of Maryland, College Park, MD, United States;University of Maryland Institute for Advanced Computer Studies, University of Maryland, College Park, MD, United States;

关键词: Vibrio cholerae;    non-O1/non-O139;    Haiti;    plasmid;    cholera;   

DOI  :  10.3389/fmicb.2017.02283

来源: DOAJ

【 摘 要 】

We report the complete sequence of two novel plasmids, pSDH-1 and pSDH-2, isolated from clinical Vibrio cholerae non-O1/non-O139 during the early phase of the 2010 Haitian cholera epidemic. Plasmids were revealed by employing single-cell genomics and their genome content suggests self-mobilization and, for pSDH-2, a toxin-antitoxin (TA) system for plasmid stabilization was identified. The putative origin of replication of pSDH-2 was mapped suggesting it replicates following the ColE1 model of plasmid replication. pSDH-1 and pSDH-2 were widespread among environmental V. cholerae non-O1/non-O139 with variable prevalence in four Haitian Departments. pSDH-2 was the most common element, either alone or with pSDH-1. The two plasmids detection adds to the composite scenario of mobile genetic elements (MGEs) observed in V. cholerae in Haiti. The role these small cryptic plasmids circulating in Vibrio spp. play in bacterial fitness or pathogenicity merits further investigation.

【 授权许可】

Unknown