Overexpression of OsMed16 Inhibits the Growth of Rice and Causes Spontaneous Cell Death

Genes

Xiaoyan Tang¹  Wei Yan¹  Zhufeng Chen²  Guangzhe Yang³  Jie Jiang³  Zhigang Wang³  Yafeng Xin³  Jixing Xia³ 

[1] Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China;Shenzhen Institute of Molecular Crop Design, Shenzhen 518107, China;State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, China;

关键词: Mediator subunit;    OsMed16;    Oryza sativa;    spontaneous cell death;    defense response;   

DOI  :  10.3390/genes12050656

来源: DOAJ

【 摘 要 】

The Mediator complex transduces information from the DNA-bound transcription factors to the RNA polymerase II transcriptional machinery. Research on plant Mediator subunits has primarily been performed in Arabidopsis, while very few of them have been functionally characterized in rice. In this study, the rice Mediator subunit 16, OsMed16, was examined. OsMed16 encodes a putative protein of 1301 amino acids, which is longer than the version previously reported. It was expressed in various rice organs and localized to the nucleus. The knockout of OsMed16 resulted in rice seedling lethality. Its overexpression led to the retardation of rice growth, low yield, and spontaneous cell death in the leaf blade and sheath. RNA sequencing suggested that the overexpression of OsMed16 altered the expression of a large number of genes. Among them, the upregulation of some defense-related genes was verified. OsMed16 can regulate the expression of a wealth of genes, and alterations in its expression have a profound impact on plant growth, development, and defense responses in rice.

【 授权许可】

Unknown