Identification of the NADPH Oxidase (Nox) Subtype and the Source of Superoxide Production in the Micturition Centre

Biology

Michael R. Ruggieri¹  Patrizia Camelliti²  Ayse Gurpinar²  Maxwell Roberts²  Changhao Wu²  Qin Wu³ 

[1] Department of Anatomy & Cell Biology, Temple University, Philadelphia, PA 19122, USA;School of Biosciences and Medicine, University of Surrey, Guildford GU2 7XH, UK;School of Medicine, Jiangsu Vocational College of Medicine, Yancheng 224005, China;

关键词: NADPH oxidase (Nox);    periaqueductal gray (PAG);    pontine micturition centre (PMC);    reactive oxygen species (ROS);    bladder;    aging;   

DOI  :  10.3390/biology11020183

来源: DOAJ

【 摘 要 】

Oxidative inflammatory damage to specialised brain centres may lead to dysfunction of their associated peripheral organs, such as the bladder. However, the source of reactive oxygen species (ROS) in specific brain regions that regulate bladder function is poorly understood. Of all ROS-generating enzymes, the NADPH oxidase (Nox) family produces ROS as its sole function and offers an advantage over other enzymes as a drug-targetable molecule to selectively control excessive ROS. We investigated whether the Nox 2 subtype is expressed in the micturition regulatory periaqueductal gray (PAG) and Barrington’s nucleus (pontine micturition centre, PMC) and examined Nox-derived ROS production in these structures. C57BL/6J mice were used; PAG, PMC, cardiac tissue, and aorta were isolated. Western blot determined Nox 2 expression. Lucigenin-enhanced chemiluminescence quantified real-time superoxide production. Western blot experiments demonstrated the presence of Nox 2 in PAG and PMC. There was significant NADPH-dependent superoxide production in both brain tissues, higher than that in cardiac tissue. Superoxide generation in these brain tissues was significantly suppressed by the Nox inhibitor diphenyleneiodonium (DPI) and also reduced by the Nox-2 specific inhibitor GSK2795039, comparable to aorta. These data provide the first evidence for the presence of Nox 2 and Nox-derived ROS production in micturition centres.

【 授权许可】

Unknown