Hydrothermal Effect on Mechanical Properties of Nephila pilipes Spidroin

Polymers

Hsuan-Chen Wu¹  ThomasChung-Kuang Yang²  I-Min Tso³  Liang-Yu Chang⁴  Chieh-Yun Hsu⁴  Aditi Pandey⁴  Jen-Chang Yang⁴  Hwo-Shuenn Sheu⁵ 

[1] Department of Biochemical Science and Technology, National Taiwan University, Taipei 11052, Taiwan;Department of Chemical Engineering and Biotechnology, National Taipei University of Technology, Taipei 11052, Taiwan;Department of Life Science, Tunghai University, Taichung 400, Taiwan;Graduate Institute of Nanomedicine and Medical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei 11052, Taiwan;National Synchrotron Radiation Research Center, Hsinchu 300, Taiwan;

关键词: spider silk;    secondary structures;    hydrothermal treatment;    strength;    Nephila pilipes;   

DOI  :  10.3390/polym12051013

来源: DOAJ

【 摘 要 】

The superlative mechanical properties of spider silk and its conspicuous variations have instigated significant interest over the past few years. However, current attempts to synthetically spin spider silk fibers often yield an inferior physical performance, owing to the improper molecular interactions of silk proteins. Considering this, herein, a post-treatment process to reorganize molecular structures and improve the physical strength of spider silk is reported. The major ampullate dragline silk from Nephila pilipes with a high β-sheet content and an adequate tensile strength was utilized as the study material, while that from Cyrtophora moluccensis was regarded as a reference. Our results indicated that the hydrothermal post-treatment (50–70 °C) of natural spider silk could effectively induce the alternation of secondary structures (random coil to β-sheet) and increase the overall tensile strength of the silk. Such advantageous post-treatment strategy when applied to regenerated spider silk also leads to an increment in the strength by ~2.5–3.0 folds, recapitulating ~90% of the strength of native spider silk. Overall, this study provides a facile and effective post-spinning means for enhancing the molecular structures and mechanical properties of as-spun silk threads, both natural and regenerated.

【 授权许可】

Unknown