Distinguishing Between Nile Tilapia Strains Using a Low-Density Single-Nucleotide Polymorphism Panel

Frontiers in Genetics

Ma. Jodecel C. Danting¹  Ravelina R. Velasco³  John A. H. Benzie⁴  Curtis E. Lind⁵  Benoy K. Barman⁵  Matthew G. Hamilton⁵ 

[1] Bureau of Fisheries and Aquatic Resources, National Freshwater Fisheries Technology Center, Central Luzon State University Compound, Science City of Munoz, Philippines;Department of Aquatic Resources, Ecology and Management, College of Fisheries, Central Luzon State University, Muñoz, Philippines;Freshwater Aquaculture Center, Central Luzon State University, Muñoz, Philippines;School of Biological Earth and Environmental Sciences, University College Cork, Cork, Ireland;WorldFish, Penang, Malaysia;

关键词: aquaculture;    nile tilapia (Oreochromis niloticus);    genetic improvement;    single nucleotide polymorphism (SNP);    discriminant analysis of principal components (DAPC);    strain identification;   

DOI  :  10.3389/fgene.2020.594722

来源: DOAJ

【 摘 要 】

Nile tilapia (Oreochromis niloticus) is among the most important finfish in aquaculture, particularly in Asia. Numerous genetically improved strains of Nile tilapia have been developed and disseminated through formal and informal channels to hatcheries, many of which operate at a relatively small scale in developing countries. The primary objective of this study was to assess the extent to which molecular genetic tools can identify different and interrelated strains of Nile tilapia in Bangladesh and the Philippines, two globally significant producers. A tool was developed using a low-density panel of single-nucleotide polymorphisms (SNPs), genotyping-by-sequencing and discriminant analysis of principal components (DAPC). When applied to 2,057 samples from 205 hatcheries in Bangladesh and the Philippines, for hatcheries where the hatchery-identified strain was one of the sampled core populations used to develop the tool, hatchery-identified and DAPC-assigned hatchery-level strains were in agreement in 74.1% of cases in Bangladesh and 80.6% of cases in the Philippines. The dominant hatchery-identified and DAPC-assigned strains were GIFT, in Bangladesh, and GET-ExCEL—a composite strain partially derived from GIFT—in the Philippines.

【 授权许可】

Unknown