A compartmentalized signaling network mediates crossover control in meiosis

eLife

Regina Rillo-Bohn¹  Abby F Dernburg²  Simone Köhler³  Liangyu Zhang⁴ 

[1] Biological Systems and Engineering Division, Lawrence Berkeley National Laboratory, Berkeley, United States;California Institute for Quantitative Biosciences, Berkeley, United States;Howard Hughes Medical Institute, Chevy Chase, United States;Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, United States;

关键词: meiosis;    crossover control;    synaptonemal Complex;    RING finger ligase;    homologous recombination;    Turing pattern;   

DOI  :  10.7554/eLife.30789

来源: DOAJ

【 摘 要 】

During meiosis, each pair of homologous chromosomes typically undergoes at least one crossover (crossover assurance), but these exchanges are strictly limited in number and widely spaced along chromosomes (crossover interference). The molecular basis for this chromosome-wide regulation remains mysterious. A family of meiotic RING finger proteins has been implicated in crossover regulation across eukaryotes. Caenorhabditis elegans expresses four such proteins, of which one (ZHP-3) is known to be required for crossovers. Here we investigate the functions of ZHP-1, ZHP-2, and ZHP-4. We find that all four ZHP proteins, like their homologs in other species, localize to the synaptonemal complex, an unusual, liquid crystalline compartment that assembles between paired homologs. Together they promote accumulation of pro-crossover factors, including ZHP-3 and ZHP-4, at a single recombination intermediate, thereby patterning exchanges along paired chromosomes. These proteins also act at the top of a hierarchical, symmetry-breaking process that enables crossovers to direct accurate chromosome segregation.

【 授权许可】

Unknown