The immune-modulation and gut microbiome structure modification associated with long-term dietary supplementation of Lactobacillus rhamnosus using 16S rRNA sequencing analysis

Journal of Functional Foods

Dapeng Ding¹  Abdo Meyiah²  Xiaomeng Ren²  Xinxiu Ren²  Yaser Gamallat²  Yufang Ma²  Abdullah Shopit²  Meiqi Li²  Siyuan Song²  Luhan Xie²  Yazeed Jamalat²  Yi Xin²  Haithm Mousa³  Bashir Ahmad⁴ 

[1] Corresponding authors.;Department of Biotechnology, College of Basic Medical Science, Dalian Medical University, Dalian, Liaoning 116044, China;Department of Clinical Laboratory Medicine, First Affiliated Hospital of Dalian Medical University, Dalian 116011, China;Department of Pathology and Pathophysiology, College of Basic Medical Science, Dalian Medical University, Dalian, Liaoning 116044, China;

关键词: Lactobacillus rhamnosus (LBR);    Probiotic;    Gut microbiome;    16S rRNA;    KEGG orthologs;   

DOI  :  

来源: DOAJ

【 摘 要 】

Probiotic dietary supplementation plays a crucial role in shaping gut microbiota and boost immune-responses. We herein investigated the potential effect of long-term dietary-supplementation of Lactobacillus rhamnosus (LBR) on gut microbiome structure, metabolome functions, and serum-profile. Our results revealed, LBR long-term supplementation reduces the body weight gain, ameliorates serum cytokines and attenuates serum lipoproteins profile. Moreover, LBR enhances biofilms-aerobics. Gut microbiome 16S rRNA analysis revealed LBR intervention significantly increased the relative abundance of Limnobacter, Turicibacterales, Enterococcus, and Vagococcus. On the contrary, the abundance of Helicobacteraceae, Rikenellaceae, Roseburia, Dorea, Anaerostipes, Coprococcus, Lachnospira, Oscillospira, Ruminococcaceae, Clotridiales, S24_7, Bacteroidia and Bacteroidetes found decreased in LBR treated group. Functional comparison revealed LBR exhibited higher level of energy production and conversion, amino acid transport and metabolism functions. Our findings demonstrate that the LBR dietary supplementation found to be beneficially modulating the immune-response, enhances lipoprotein metabolism, and ameliorates the microbial composition structure and functions.

【 授权许可】

Unknown