The Application and Interpretation of IgG Avidity and IgA ELISA Tests to Characterize Zika Virus Infections

Viruses

Fátima Amaro¹  Ana Vázquez²  María P. Sánchez-Seco²  Fernando De Ory²  Jesús De La Fuente²  Teodora Minguito²  Maria T. Luz³  Líbia Zé-Zé³  Maria J. Alves⁴ 

[1] European Programme for Public Health Microbiology Training (EUPHEM), European Centre for Disease Prevention and Control (ECDC), 17165 Solna, Sweden;National Centre for Microbiology, Institute of Health Carlos III, 28220 Majadahonda, Spain;National Institute of Health Doutor Ricardo Jorge, Centre for Vectors and Infectious Diseases Research 2965-575 Águas de Moura, Portugal;Virored-Network for Emerging Viruses;

关键词: Zika virus;    dengue virus;    secondary infections;    cross-reactions;    IgA;    IgG avidity tests;   

DOI  :  10.3390/v11020179

来源: DOAJ

【 摘 要 】

In the absence of viremia, the diagnostics of Zika virus (ZIKV) infections must rely on serological techniques. In order to improve the serological diagnosis of ZIKV, ZIKV-IgA and ZIKV-IgG avidity assays were evaluated. Forty patients returning from ZIKV endemic areas, with confirmed or suspected ZIKV infections were studied. Samples were classified as early acute, acute and late acute according to the number of days post illness onset. Low avidity IgG was only detected at acute and late acute stages and IgA mostly at the early acute and acute stages. The date of sampling provides useful information and can help to choose the best technique to use at a determined moment in time and to interpret low avidity IgG and IgA results, improving the serological diagnosis of ZIKV.

【 授权许可】

Unknown