Naringenin-Functionalized Multi-Walled Carbon Nanotubes: A Potential Approach for Site-Specific Remote-Controlled Anticancer Delivery for the Treatment of Lung Cancer Cells

International Journal of Molecular Sciences

Margreet Morsink¹  ElianaB. Souto²  AndréL. S. Santos³  LeandroS. Sangenito³  Ronny Priefer⁴  JulianaC. Cardoso⁵  RenataP. Morais⁵  Patrícia Severino⁵  MarceloC. Mendonça⁵  GabrielleB. Novais⁵ 

[1] Center for Biomedical Engineering, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, 65 Landsdowne Street, Cambridge, MA 02139, USA;Department of Pharmaceutical Technology, Faculty of Pharmacy, University of Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal;Federal University of Rio de Janeiro (UFRJ), 373, Carlos Chagas Filho Ave, Cidade Universitária da Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-901, Brazil;School of Pharmacy, Massachusetts College of Pharmacy and Health Sciences, 179 Longwood Avenue, Boston, MA 02115, USA;Tiradentes University (UNIT), 300, Murilo Dantas Ave, Farolândia 49032-490, Brazil;

关键词: carbon nanotubes;    naringenin;    flavanones;    functionalization;    antineoplastic agents;    lung cancer;   

DOI  :  10.3390/ijms21124557

来源: DOAJ

【 摘 要 】

Multi-walled carbon nanotubes functionalized with naringenin have been developed as new drug carriers to improve the performance of lung cancer treatment. The nanocarrier was characterized by Transmission Electron Microscopy (TEM), Fourier-Transform Infrared Spectroscopy (FTIR), X-ray photoelectron spectroscopy, Raman Spectroscopy, and Differential Scanning Calorimetry (DSC). Drug release rates were determined in vitro by the dialysis method. The cytotoxic profile was evaluated using the MTT assay, against a human skin cell line (hFB) as a model for normal cells, and against an adenocarcinomic human alveolar basal epithelial (A569) cell line as a lung cancer in vitro model. The results demonstrated that the functionalization of carbon nanotubes with naringenin occurred by non-covalent interactions. The release profiles demonstrated a pH-responsive behavior, showing a prolonged release in the tumor pH environment. The naringenin-functionalized carbon nanotubes showed lower cytotoxicity on non-malignant cells (hFB) than free naringenin, with an improved anticancer effect on malignant lung cells (A549) as an in vitro model of lung cancer.

【 授权许可】

Unknown