Effects of chromium stress on the rhizosphere microbial community composition of Cyperus alternifolius

Ecotoxicology and Environmental Safety

Hongyu Luo¹  Sixi Zhu²  Qian Tang²  Baichun Wang³  Wujiang Li³ 

[1] Corresponding author at: College of Eco-environment Engineering, Guizhou Minzu University, Guiyang 550025, China.;Institute of Karst Wetland Ecology, Guizhou Minzu University, Guiyang 550025, China;College of Eco-environment Engineering, Guizhou Minzu University, Guiyang 550025, China;

关键词: Chromium stress;    Rhizosphere microbial community;    Cyperus alternifolius;    16s rRNA gene sequencing;    Phytoremediation;   

DOI  :  

来源: DOAJ

【 摘 要 】

Wetland plants are often used as the main body of soil, and the rhizosphere is a hot spot migration and transformation. Response mechanism to rhizosphere microorganisms on chromium(Cr) stressing could help improve the phytoremediation system. Cyperus alternifolius(CA) is selected as the research object by Cr-stress treatments and uncontaminated treatments with different cultivated pattern, included sole cultivated pattern(CAI), two-cultivated pattern (CAII), three-cultivated pattern (CAIII), and the un-planted blank samples (CK). 16s rRNA gene sequencing and metagenomic sequencing are performed to measure rhizosphere microbial community. And Five common enzymes in rhizosphere soils were observed: β-1,4-glucosidase (BG), β-N-acetylglucosaminidase (NAG), β-1,4-xylosidase (BX), cellobiohydrolase (CBH) and Leucine amino peptidase (LAP) in the rhizosphere. The results show that Gammaproteobacteria, Actinobacteria, Alphaproteobacteria, Gemmatimonadetes, Deltaproteobacteria are top five (63.97%) of the total sequence number. Wetland plants enriched a large amount of soil Cr in themselves, and the rhizosphere microorganisms don’t show significant difference in community structure after affecting. 10.48% variation of microbial community is caused by Cr-stress. Acidovorax showed a great potential for chromium resistance. BX involvement in tolerance processes indirectly affects microbial communities (P < 0.01), there is a strong linear relationship between enzyme activity and the plants accumulating Cr and microbial community within 15.58% variant. The material accumulation and microbial quantity of CAIII are relatively low, but high biodiversity remains after affecting. These results provide references for in-depth understanding of rhizosphere microbial response to heavy metal pollution in wetland phytoremediation and interaction between wetland plants and rhizosphere microorganisms.

【 授权许可】

Unknown