Full-Genome Sequences and Phylogenetic Analysis of Archived Danish European Bat Lyssavirus 1 (EBLV-1) Emphasize a Higher Genetic Resolution and Spatial Segregation for Sublineage 1a

Viruses

DeniseA. Marston¹  AnthonyR. Fooks¹  Lorraine McElhinney¹  ConradM. Freuling²  Martin Beer³  Sten Calvelage³  Dirk Höper³  Thomas Müller⁴  Stefan Finke⁴  ThomasBruun Rasmussen⁵ 

[1] Animal & Plant Health Agency, WHO Collaborating Centre for Rabies Surveillance and Research, OIE Reference Laboratory for Rabies, Weybridge KT15 3NB, Surrey, UK;Central Duties, Friedrich-Loeffler-Institut (FLI), 17493 Greifswald-Insel Riems, Germany;Institute of Diagnostic Virology, Friedrich-Loeffler-Institut (FLI), 17493 Greifswald-Insel Riems, Germany;Institute of Molecular Virology and Cell Biology, Friedrich-Loeffler-Institut (FLI), WHO Collaborating Centre for Rabies Surveillance and Research, OIE Reference Laboratory for Rabies, 17493 Greifswald-Insel Riems, Germany;Virus & Mikrobiologisk Specialdiagnostik, Statens Serum Institut, 2300 Copenhagen, Denmark;

关键词: European bat lyssavirus 1 (EBLV-1);    next generation sequencing (NGS);    bats;    full-genome sequencing;    zoonoses;   

DOI  :  10.3390/v13040634

来源: DOAJ

【 摘 要 】

European bat lyssavirus type 1 (EBLV-1) is the causative agent for almost all reported rabies cases found in European bats. In recent years, increasing numbers of available EBLV-1 full genomes and their phylogenetic analyses helped to further elucidate the distribution and genetic characteristics of EBLV-1 and its two subtypes, namely EBLV-1a and EBLV-1b. Nonetheless, the absence of full-genome sequences from regions with known detections of EBLV-1 still limit the understanding of the phylogeographic relations between viruses from different European regions. In this study, a set of 21 archived Danish EBLV-1 samples from the years 1985 to 2009 was processed for the acquisition of full-genome sequences using a high-throughput sequencing approach. Subsequent phylogenetic analysis encompassing all available EBLV-1 full genomes from databases revealed the Danish sequences belong to the EBLV-1a subtype and further highlighted the distinct, close phylogenetic relationship of Danish, Dutch and German isolates in this region. In addition, the formation of five putative groups nearly exclusively formed by Danish isolates and the overall increased resolution of the EBLV-1a branch indicate a higher genetic diversity and spatial segregation for this sublineage than was previously known. These results emphasize the importance of phylogenetic analyses of full-genome sequences of lyssaviruses for genetic geography.

【 授权许可】

Unknown