Agro-Morphological, Microanatomical and Molecular Cytogenetic Characterization of the Medicinal Plant Chelidonium majus L

Plants

ElenaA. Konyaeva¹  FirdausM. Hazieva¹  AlexanderI. Morozov¹  TatianaE. Samatadze²  OlgaV. Muravenko²  AlexandraV. Amosova²  OlgaYu. Yurkevich²  SvyatoslavA. Zoshchuk² 

[1] All-Russian Scientific Research Institute of Medicinal and Aromatic Plants, 7 Green St, 117216 Moscow, Russia;Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, 32 Vavilov St, 119991 Moscow, Russia;

关键词: Chelidonium majus L. subsp. majus;    bio-morphology;    phytotomy of leaves and flowers;    meiosis;    karyotype;    fluorescence in situ hybridization (FISH);   

DOI  :  10.3390/plants9101396

来源: DOAJ

【 摘 要 】

Chelidonium majus L. is a medicinal plant well-known as a valuable source of isoquinoline alkaloids, which has a variety of pharmacological properties including anti-viral and anti-bacterial effects. However, considerable intraspecific bio-morphological variability in C. majus complicates raw material identification and verification. For the first time, we have brought into cultivation five populations of C. majus subsp. majus originated from different regions, and performed their agro-morphological, microanatomical and molecular cytogenetic characterization. All examined populations produced high seed (18.6–19.9 kg/ha) and raw material (0.84–1.08 t/ha) yields; total alkaloid contents were within 0.30–0.38%. Nevertheless, significant differences in plant morphology and yield-contributing traits were observed. The performed microanatomical analysis of leaves and flowers in double- and normal-flowered plants revealed micro-diagnostic features (including tissue topography, types of stomata, laticifers, structure of leaf mesophyll, hairs, sepals and petals) important for identification of C. majus raw materials. The analysis of chromosome morphology, DAPI-banding patterns, FISH mapping of 45S and 5S rDNA and also chromosome behavior in meiosis allowed us to identify for the first time all chromosomes in karyotypes and confirm relative genotype stability of the studied plants. Our findings indicate that the examined C. majus populations can be used in further breeding programs.

【 授权许可】

Unknown