Single‐cell transcriptomics analysis of mild cognitive impairment in World Trade Center disaster responders

Alzheimer’s & Dementia: Diagnosis, Assessment & Disease Monitoring

Pei‐Fen Kuan¹  Chang Che¹  Sean Clouston²  Benjamin J. Luft³  Xiaohua Yang³  Samuel Gandy⁴  Evelyn Bromet⁵  Roman Kotov⁵ 

[1] Department of Applied Mathematics and Statistics Stony Brook University Stony Brook New York USA;Department of Family and Preventive Medicine Stony Book University Stony Brook New York USA;Department of Medicine Stony Brook University Stony Brook New York USA;Department of Neurology Icahn School of Medicine at Mount Sinai New York New York USA;Department of Psychiatry Stony Book University Stony Brook New York USA;

关键词: CITE‐Seq;    mild cognitive impairment;    World Trade Center;   

DOI  :  10.1002/dad2.12154

来源: DOAJ

【 摘 要 】

Abstract Introduction Recent research has found that World Trade Center (WTC) responders in their mid‐50s have an elevated prevalence of mild cognitive impairment (MCI) that is associated with neural degeneration and subcortical thinning. This article extends our understanding of the molecular complexity of MCI through gene expression profiling of blood. Methods The transcriptomics of 40 male WTC responders were profiled across two cohorts (discovery: nine MCI and nine controls; replication: 11 MCI and 11 controls) using CITE‐Seq at single‐cell resolution in blood. Results Comparing the transcriptomic signatures across seven major cell subpopulations, the largest differences were observed in monocytes in which 226 genes were differentially expressed. Pathway analysis on the genes unique to monocytes identified processes associated with cerebral immune response. Discussion Our findings suggested monocytes may constitute a key cell type to target in blood‐based biomarker studies for early detection of risk of MCI and development of new interventions.

【 授权许可】

Unknown