Green Synthesis and Characterization of Zinc Oxide Nanoparticles Using Eucalyptus globules and Their Fungicidal Ability Against Pathogenic Fungi of Apple Orchards

Biomolecules

Kalyanaraman Rajagopal¹  Krishnan Venugopal²  Narasimhamurthy Konappa³  Savitha De Britto⁴  HosurGnanaprakash Pushpalatha⁵  Sudisha Jogaiah⁵  ArakereC. Udayashankar⁵  Nagaraja Geetha⁵  Boregowda Nandini⁶  Hilal Ahmad⁷ 

[1] Sciences for Women, Elayampalayam, Tiruchengode 637205, Namakkal Dist., Tamilnadu, India;;Department of Biochemistry, Vivekanandha College of Arts &Department of Botany, Maharani’s Science College for Women, JLB Road, Mysuru 570 001, Karnataka, India;Department of Botany, Ramakrishna Mission Vivekananda College, Chennai 600004, Tamilnadu, India;Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru 570 006, Karnataka, India;Laboratory of Plant Healthcare and Diagnostics, PG Department of Biotechnology and Microbiology, Karnataka University, Dharwad 580003, Karnataka, India;School of Life Sciences, Department of Biotechnology, Vels University, Pallavarm, Chennai 600117, Tamilnadu, India;

关键词: eucalyptus globules;    zinc nanoparticles;    apple;    antifungal activity;    ftir;    sem;    tem;    fruit crops;   

DOI  :  10.3390/biom10030425

来源: DOAJ

【 摘 要 】

Eucalyptus globules belonging to the Myrtaceae family was explored for the synthesis of zinc oxide nanoparticles and for biological applications. The aqueous extract of the synthesized zinc nanoparticles (ZnNPs) was characterized using UV-visible spectrophotometer, FTIR, SEM and TEM. The aqueous broth was observed to be an efficient reducing agent, leading to the rapid formation of ZnNPs of varied shapes with sizes ranging between 52−70 nm. In addition, antifungal activity of the biosynthesized ZnNPs was evaluated against major phytopathogens of apple orchards. At 100 ppm of ZnNPs, the fungal growth inhibition rate was found to be 76.7% for Alternaria mali, followed by 65.4 and 55.2% inhibition rate for Botryosphaeria dothidea and Diplodia seriata, respectively. The microscopic observations of the treated fungal plates revealed that ZnNPs damages the topography of the fungal hyphal layers leading to a reduced contraction of hyphae. This considerable fungicidal property of ZnNPs against phytopathogenic fungi can have a tremendous impact on exploitation of ZnNPs for fungal pest management and ensure protection in fruit crops.

【 授权许可】

Unknown