Instrument-Free and Visual Detection of Salmonella Based on Magnetic Nanoparticles and an Antibody Probe Immunosensor

International Journal of Molecular Sciences

Haiming Luo¹  Nanxin Gong²  Jinyang Zhang²  Xuewei Du²  Xueshan Xia²  Liding Zhang²  Zhixin Chen²  Congjie Chen²  Yihao Song²  Yuzhu Song²  Qinqin Han² 

[1] Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and Technology, Wuhan 430074, Hubei, China;Molecular Medicine Research Center of Yunnan Province, Faculty of Life Science and Technology, Kunming University of Science and Technology, 727 Jingming South Road, Kunming 650500, China;

关键词: Salmonella;    monoclonal antibody;    magnetic nanoparticles;    HRP-probe;    immunoassay;   

DOI  :  10.3390/ijms20184645

来源: DOAJ

【 摘 要 】

Salmonella, a common foodborne pathogen, causes many cases of foodborne illness and poses a threat to public health worldwide. Immunological detection systems can be combined with nanoparticles to develop sensitive and portable detection technologies for timely screening of Salmonella infections. Here, we developed an antibody-probe-based immuno-N-hydroxysuccinimide (NHS) bead (AIB) system to detect Salmonella. After adding the antibody probe, Salmonella accumulated in the samples on the surfaces of the immuno-NHS beads (INBs), forming a sandwich structure (INB−Salmonella−probes). We demonstrated the utility of our AIB diagnostic system for detecting Salmonella in water, milk, and eggs, with a sensitivity of 9 CFU mL⁻¹ in less than 50 min. The AIB diagnostic system exhibits highly specific detection and no cross-reaction with other similar microbial strains. With no specialized equipment or technical requirements, the AIB diagnostic method can be used for visual, rapid, and point-of-care detection of Salmonella.

【 授权许可】

Unknown