Long noncoding RNA LINC01189 is associated with HCV-hepatocellular carcinoma and regulates cancer cell proliferation and chemoresistance through hsa-miR-155-5p

Annals of Hepatology

Haigang Lv¹  Lu Li¹  Fang Wang¹  Zhengshe Guo¹  Haili Wang¹  Ying Yao²  Fang Shu³  Xiaoqiang Wang⁴ 

[1] Anaesthesiology department, Honghui Hospital, Xi’an Jiaotong University, Xi’an, 710054, Shaanxi, China;Clinical laboratory, Honghui Hospital, Xi’an Jiaotong University, Xi’an, 710054 Shaanxi, China;Clinical laboratory, Third Hospital of Xi’an, 710000, Shaanxi, China;Department of Cancer Biology, City of Hope National Medical Center, Duarte, CA, 91010, USA;

关键词: HCV;    HCC;    lncRNA;    LINC01189;    hsa-miR-155-5p;   

DOI  :  

来源: DOAJ

【 摘 要 】

Introduction and Objectives: Emerging evidence has demonstrated that long noncoding RNAs (lncRNAs) may be closely associated with Hepatitis C virus (HCV) infection and the development of hepatocellular carcinoma (HCC). In this study, we investigated the expression and functions of a lncRNA, LINC01189, in HCV-associated HCC. Patients or Materials and Methods: LINC01189 expression was measured in HCC tumors, HCV-infected HCC tumors and HCV-infected HCC cells. LINC01189 was overexpressed in HCV-infected HepG2 cells to measure its function on HCV-correlated cancer proliferation. In HCC cell lines of Huh7 and Hep3B, LINC01189 was upregulated to investigate its effects on cancer cell proliferation and 5-FU chemoresistance. The competing endogenous RNA (ceRNA) target of LINC01189, human microRNA-155-5p (hsa-miR-155-5p) was probed by dual-luciferase assay and qRT-PCR. Hsa-miR-155-5p was upregulated in LINC01189-overexpessed Huh7 and Hep3B cells to investigate their epigenetic correlation on HCC development regulation. Results: LINC01189 is downregulated in HCV-infected HCC tumors and cell lines. LINC01189 overexpression inhibited HCC cancer cell proliferation and 5-FU chemoresistance. Hsa-miR-155-5p was confirmed to be a ceRNA target of LINC01189 in HCC. Upregulating hsa-miR-155-5p reversed the LINC01189-mediated inhibition on HCC proliferation and 5-FU chemoresistance. Conclusions: LINC01189 downregulation is associated with HCV infection in HCC, and it has tumor-suppressing effects on HCC development through hsa-miR-155-5p.

【 授权许可】

Unknown