Palm Oil Mill Effluent for Lipid Production by the Diatom Thalassiosira pseudonana

Fermentation

Karthick Murugan Palanisamy¹  Gaanty Pragas Maniam¹  Mohd Hasbi Ab. Rahim¹  Natanamurugaraj Govindan¹  Ahmad Ziad Sulaiman²  Yusuf Chisti³ 

[1] Algae Biotechnology Laboratory, Faculty of Industrial Sciences and Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, Gambang, Kuantan 26300, Pahang, Malaysia;Faculty of Bioengineering and Technology, Universiti Malaysia Kelantan, Jeli 17600, Kelantan, Malaysia;School of Engineering, Massey University, Private Bag 11 222, Palmerston North 4442, New Zealand ;

关键词: Thalassiosira pseudonana;    palm oil mill effluent;    diatom lipids;    algal biodiesel;   

DOI  :  10.3390/fermentation8010023

来源: DOAJ

【 摘 要 】

Biomass and lipid production by the marine centric diatom Thalassiosira pseudonana were characterized in media based on palm oil mill effluent (POME) as a source of key nutrients. The optimal medium comprised 20% by volume POME, 80 µM Na₂SiO₃, and 35 g NaCl L⁻¹ in water at pH ~7.7. In 15-day batch cultures (16:8 h/h light–dark cycle; 200 µmol photons m⁻² s⁻¹, 26 ± 1 °C) bubbled continuously with air mixed with CO₂ (2.5% by vol), the peak concentration of dry biomass was 869 ± 14 mg L⁻¹ corresponding to a productivity of ~58 mg L⁻¹ day⁻¹. The neutral lipid content of the biomass was 46.2 ± 1.1% by dry weight. The main components of the esterified lipids were palmitoleic acid methyl ester (31.6% w/w) and myristic acid methyl ester (16.8% w/w). The final biomass concentration and the lipid content were affected by the light–dark cycle. Continuous (24 h light) illumination at the above-specified irradiance reduced biomass productivity to ~54 mg L⁻¹ day⁻¹ and lipid content to 38.1%.

【 授权许可】

Unknown