Super-Resolution Microscopy Reveals Diversity of Plant Centromere Architecture

International Journal of Molecular Sciences

Pavel Neumann¹  Jiri Macas¹  Tae-Soo Jang¹  Andrea Pedrosa-Harand²  André Marques³  Stefan Heckmann⁴  Veit Schubert⁴  Ingo Schubert⁴  Andreas Houben⁴ 

[1] Biology Centre, Czech Academy of Sciences, 37005 České Budějovice, Czech Republic;Department of Botany, Federal University of Pernambuco (UFPE), Recife 50670-901, Pernambuco, Brazil;Department of Chromosome Biology, Max Planck Institute for Plant Breeding Research, 50829 Cologne, Germany;Leibniz Institute of Plant Genetics and Crop Plant Research (IPK) Gatersleben, 06466 Seeland, Germany;

关键词: CENH3;    CENP-A;    clustered centromere;    Cuscuta;    holocentromere;    Lathyrus;   

DOI  :  10.3390/ijms21103488

来源: DOAJ

【 摘 要 】

Centromeres are essential for proper chromosome segregation to the daughter cells during mitosis and meiosis. Chromosomes of most eukaryotes studied so far have regional centromeres that form primary constrictions on metaphase chromosomes. These monocentric chromosomes vary from point centromeres to so-called “meta-polycentromeres”, with multiple centromere domains in an extended primary constriction, as identified in Pisum and Lathyrus species. However, in various animal and plant lineages centromeres are distributed along almost the entire chromosome length. Therefore, they are called holocentromeres. In holocentric plants, centromere-specific proteins, at which spindle fibers usually attach, are arranged contiguously (line-like), in clusters along the chromosomes or in bands. Here, we summarize findings of ultrastructural investigations using immunolabeling with centromere-specific antibodies and super-resolution microscopy to demonstrate the structural diversity of plant centromeres. A classification of the different centromere types has been suggested based on the distribution of spindle attachment sites. Based on these findings we discuss the possible evolution and advantages of holocentricity, and potential strategies to segregate holocentric chromosomes correctly.

【 授权许可】

Unknown