Highly efficient magnetic labelling allows MRI tracking of the homing of stem cell‐derived extracellular vesicles following systemic delivery

Journal of Extracellular Vesicles

Zheng Ding¹  Senquan Liu¹  Xinge Wang²  Linzhao Cheng³  Tom Driedonks⁴  Kenneth W. Witwer⁴  Shuli Xia⁵  Daqian Zhan⁵  Jeff W.M. Bulte⁶  Yigang Pei⁶  Zheng Han⁶  Guanshu Liu⁶  Peter C.M. vanZijl⁶  Robert G. Weiss⁶  Yuguo Li⁶ 

[1] Cellular Imaging Section and Vascular Biology Program Institute for Cell Engineering Johns Hopkins University School of Medicine Baltimore Maryland USA;Department of Bioengineering University of Illinois at Chicago Chicago Illinois USA;Department of Medicine Johns Hopkins University School of Medicine Baltimore Maryland USA;Department of Molecular and Comparative Pathobiology Johns Hopkins University School of Medicine Baltimore Maryland USA;Department of Neurology Hugo W. Moser Research Institute at Kennedy Krieger Baltimore Maryland USA;Russell H. Morgan Department of Radiology Johns Hopkins University School of Medicine Baltimore Maryland USA;

关键词: acute kidney injury;    extracellular vesicle;    iPSC;    MRI;    myocardial injury;    stem cell;   

DOI  :  10.1002/jev2.12054

来源: DOAJ

【 摘 要 】

Abstract Human stem‐cell‐derived extracellular vesicles (EVs) are currently being investigated for cell‐free therapy in regenerative medicine applications, but the lack of noninvasive imaging methods to track EV homing and uptake in injured tissues has limited the refinement and optimization of the approach. Here, we developed a new labelling strategy to prepare magnetic EVs (magneto‐EVs) allowing sensitive yet specific MRI tracking of systemically injected therapeutic EVs. This new labelling strategy relies on the use of ‘sticky’ magnetic particles, namely superparamagnetic iron oxide (SPIO) nanoparticles coated with polyhistidine tags, to efficiently separate magneto‐EVs from unencapsulated SPIO particles. Using this method, we prepared pluripotent stem cell (iPSC)‐derived magneto‐EVs and subsequently used MRI to track their homing in different animal models of kidney injury and myocardial ischemia. Our results showed that iPSC‐derived EVs preferentially accumulated in the injury sites and conferred substantial protection. Our study paves a new pathway for preparing highly purified magnetic EVs and tracking them using MRI towards optimized, systemically administered EV‐based cell‐free therapies.

【 授权许可】

Unknown