Synthesis of NIR-II Absorbing Gelatin Stabilized Gold Nanorods and Its Photothermal Therapy Application against Fibroblast Histiocytoma Cells

Pharmaceuticals

Oluwatobi Samuel Oluwafemi¹  Thabang Calvin Lebepe¹  Adewale Oladipo¹  Sundararajan Parani¹  Vuyelwa Ncapayi¹  Rodney Maluleke¹  Grace It Mwad Mbaz¹  Sandile Phinda Songca²  Tetsuya Kodama³ 

[1] Department of Chemical Sciences, Doornfontein Campus, University of Johannesburg, Johannesburg 2028, South Africa;Department of Chemistry, University of KwaZulu-Natal, Private Bag X 54001, Durban 4000, South Africa;Graduate School of Biomedical Engineering, Tohoku University, 4-1 Seiryo, Aoba, Sendai 980-8575, Japan;

关键词: gold nanorods;    gelatin;    near-infrared;    stability;    cytotoxicity;    photothermal therapy;   

DOI  :  10.3390/ph14111137

来源: DOAJ

【 摘 要 】

The excellent photothermal properties of gold nanorods (Au-NRs) make them one of the most researched plasmonic photothermal nanomaterials. However, their biological applications have been hampered greatly due to surfactant-induced cytotoxicity. We herein report a simple synthesis of highly biocompatible gelatin stabilized Au-NRs (gelatin@Au-NRs) to address this issue. The optical and structural properties of the as-synthesized gelatin@Au-NRs were investigated by Zetasizer, Ultraviolet-Visible-Near Infrared (UV-Vis-NIR) spectroscopy, high-resolution transmission electron microscopy (HR-TEM), and Fourier transform infrared spectroscopy (FTIR). The as-synthesized gelatin@Au-NRs were highly crystalline and rod-like in shape with an average length and diameter of 66.2 ± 2.3 nm and 10 ± 1.6 nm, respectively. The as-synthesized gelatin@Au-NRs showed high stability in common biological media (phosphate buffer saline and Dulbecco’s Modified Eagle’s Medium) compared to CTAB capped Au-NRs. Similarly, the gelatin@Au-NRs showed an improved heat production and outstanding cell viability against two different cancer cell lines; KM-Luc/GFP (mouse fibroblast histiocytoma cell line) and FM3A-Luc (breast carcinoma cell line) compared to CTAB capped Au-NRs and PEG@Au-NRs. An in vitro photothermal therapy study against KM-Luc/GFP showed that gelatin@Au-NRs effectively destroys the cancer cells.

【 授权许可】

Unknown