Sleep Deprivation Aggravates Cognitive Impairment by the Alteration of Hippocampal Neuronal Activity and the Density of Dendritic Spine in Isoflurane-Exposed Mice

Frontiers in Behavioral Neuroscience

Ran Ding¹  Sheng Wei³  Hui Shen⁴  Xi Dong⁵  Qingyan Jiao⁵  Yuanyuan Li⁵  Cunle Guo⁵  Naqi Lian⁶  Kai Zhang⁶  Yonghao Yu⁶ 

[1] Chinese Institute for Brain Research, Beijing (CIBR), Beijing, China;Department of Anesthesia, Tianjin Medical University General Hospital, Tianjin, China;Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan, China;Institute of Neurology, Tianjin Medical University General Hospital, Tianjin, China;Laboratory of Neurobiology, School of Biomedical Engineering, Tianjin Medical University, Tianjin, China;Tianjin Institute of Anesthesiology, Tianjin, China;

关键词: isoflurane;    sleep deprivation;    short-term memory;    long-term memory;    Ca2+ signals;    dendritic spine;   

DOI  :  10.3389/fnbeh.2020.589176

来源: DOAJ

【 摘 要 】

Isoflurane contributes to cognitive deficits when used as a general anesthetic, and so does sleep deprivation (SD). Patients usually suffer from insomnia before an operation due to anxiety, fear, and other factors. It remains unclear whether preoperative SD exacerbates cognitive impairment induced by isoflurane. In this study, we observed the effects of pretreated 24-h SD in adult isoflurane-exposed mice on the cognitive behaviors, the Ca2+ signals of dorsal hippocampal CA1 (dCA1) neurons in vivo with fiber photometry, and the density of dendritic spines in hippocampal neurons. Our results showed that in cognitive behavior tasks, short-term memory damages were more severe with SD followed by isoflurane exposure than that with SD or isoflurane exposure separately, and interestingly, severe long-term memory deficits were induced only by SD followed by isoflurane exposure. Only the treatment of SD followed by isoflurane exposure could reversibly decrease the amplitude of Ca2+ signals when mice were freely moving and increase the duration of Ca2+ signals during the long-term memory behavior test. The density of dendritic spines with both SD and isoflurane exposure was lower than that with SD alone. This study suggests that SD should be avoided preoperatively in patients undergoing elective surgery under isoflurane anesthesia.

【 授权许可】

Unknown