Genome Engineering Technology for Durable Disease Resistance: Recent Progress and Future Outlooks for Sustainable Agriculture

Frontiers in Plant Science

Lei Zhou¹  Muhammad Imran²  Amjad Hussain³  Mohsin Ali³  Muhammad Aamir Sohail³  Xiukang Wang⁴  Sunny Ahmar⁶  Hakim Manghwar⁷  Chenjie Yu⁸  Dyaaaldin Abdalmegeed⁸  Qurban Ali⁸  Muhammad Riaz⁹  Muhammad Furqan Ashraf^1,11 

[1] 0Lushan Botanical Garden, Chinese Academy of Sciences, Jiujiang, China;College of Life Sciences, Yan’an University, Yan’an, China;College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, China;Department of Botany and Microbiology, Faculty of Science, Tanta University, Tanta, Egypt;F University, Hangzhou, China;Institute of Biology, Biotechnology, and Environmental Protection, Faculty of Natural Sciences, University of Silesia, Katowice, Poland;Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Agriculture, South China Agriculture University, Guangzhou, China;Key Laboratory of Monitoring and Management of Crop Disease and Pest Insects, College of Plant Protection, Ministry of Education, Nanjing Agricultural University, Nanjing, China;State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Root Biology Center, College of Natural Resources and Environment, South China Agricultural University, Guangzhou, China;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural Sciences, Hangzhou, China;;State Key Laboratory of Subtropical Silviculture, Zhejiang A&

关键词: plant pathogen;    genome editing;    CRISPR-Cas system;    pesticide;    disease resistance;   

DOI  :  10.3389/fpls.2022.860281

来源: DOAJ

【 摘 要 】

Crop production worldwide is under pressure from multiple factors, including reductions in available arable land and sources of water, along with the emergence of new pathogens and development of resistance in pre-existing pathogens. In addition, the ever-growing world population has increased the demand for food, which is predicted to increase by more than 100% by 2050. To meet these needs, different techniques have been deployed to produce new cultivars with novel heritable mutations. Although traditional breeding continues to play a vital role in crop improvement, it typically involves long and laborious artificial planting over multiple generations. Recently, the application of innovative genome engineering techniques, particularly CRISPR-Cas9-based systems, has opened up new avenues that offer the prospects of sustainable farming in the modern agricultural industry. In addition, the emergence of novel editing systems has enabled the development of transgene-free non-genetically modified plants, which represent a suitable option for improving desired traits in a range of crop plants. To date, a number of disease-resistant crops have been produced using gene-editing tools, which can make a significant contribution to overcoming disease-related problems. Not only does this directly minimize yield losses but also reduces the reliance on pesticide application, thereby enhancing crop productivity that can meet the globally increasing demand for food. In this review, we describe recent progress in genome engineering techniques, particularly CRISPR-Cas9 systems, in development of disease-resistant crop plants. In addition, we describe the role of CRISPR-Cas9-mediated genome editing in sustainable agriculture.

【 授权许可】

Unknown