Single Cell Multi-Omics Technology: Methodology and Application

Frontiers in Cell and Developmental Biology

Qin An¹  Brandon Trejo¹  Katherine Sheu¹  Ying Guo²  Shuxin Fan³  Youjin Hu³ 

[1] Department of Human Genetics, David Geffen School of Medicine, UCLA, Los Angeles, CA, United States;The Second Affiliated Hospital, Xiangya School of Medicine, Central South University, Changsha, China;Zhongshan Ophthalmic Center, State Key Laboratory of Ophthalmology, Sun-Ye-Sat University, Guangzhou, China;

关键词: single cell transcriptome;    single cell multi-omics profiling;    single cell epigenome;    single cell proteome;    gene regulation;    epigenetics;   

DOI  :  10.3389/fcell.2018.00028

来源: DOAJ

【 摘 要 】

In the era of precision medicine, multi-omics approaches enable the integration of data from diverse omics platforms, providing multi-faceted insight into the interrelation of these omics layers on disease processes. Single cell sequencing technology can dissect the genotypic and phenotypic heterogeneity of bulk tissue and promises to deepen our understanding of the underlying mechanisms governing both health and disease. Through modification and combination of single cell assays available for transcriptome, genome, epigenome, and proteome profiling, single cell multi-omics approaches have been developed to simultaneously and comprehensively study not only the unique genotypic and phenotypic characteristics of single cells, but also the combined regulatory mechanisms evident only at single cell resolution. In this review, we summarize the state-of-the-art single cell multi-omics methods and discuss their applications, challenges, and future directions.

【 授权许可】

Unknown