Cross-Species, Amplifiable Microsatellite Markers for Neoverrucid Barnacles from Deep-Sea Hydrothermal Vents Developed Using Next-Generation Sequencing

International Journal of Molecular Sciences

Hiromi Watanabe¹  Fumio Inagaki²  Satoshi Mitarai³  Yuichi Nakajima³  Mariia Khalturina⁴  Chuya Shinzato⁴  Nori Satoh⁴ 

[1] Department of Marine Biodiversity Research, Japan Agency for Marine-Earth Science and Technology, 2-15 Natsushima-cho, Yokosuka, Kanagawa 237-0061, Japan;Kochi Institute for Core Sample Research, Japan Agency for Marine-Earth Science and Technology, B-200 Monobe, Nankoku, Kochi 783-8502, Japan;Marine Biophysics Unit, Okinawa Institute of Science and Technology Graduate University,1919-1 Tancha, Onna, Okinawa 904-0495, Japan;Marine Genomics Unit, Okinawa Institute of Science and Technology Graduate University,1919-1 Tancha, Onna, Okinawa 904-0495, Japan;

关键词: deep-sea;    microsatellites;    Neoverruca;    population genetics;   

DOI  :  10.3390/ijms150814364

来源: DOAJ

【 摘 要 】

Barnacles of the genus Neoverruca are abundant near deep-sea hydrothermal vents of the northwestern Pacific Ocean, and are useful for understanding processes of population formation and maintenance of deep-sea vent faunas. Using next-generation sequencing, we isolated 12 polymorphic microsatellite loci from Neoverruca sp., collectedin the Okinawa Trough. These microsatellite loci revealed 2–19 alleles per locus. The expected and observed heterozygosities ranged from 0.286 to 1.000 and 0.349 to 0.935, respectively. Cross-species amplification showed that 9 of the 12 loci were successfully amplified for Neoverruca brachylepadoformis in the Mariana Trough. A pairwise FST value calculated using nine loci showed significant genetic differentiation between the two species. Consequently, the microsatellite markers we developed will be useful for further population genetic studies to elucidate genetic diversity, differentiation, classification, and evolutionary processes in the genus Neoverruca.

【 授权许可】

Unknown