Vaccines | |
The Role of Virulence Proteins in Protection Conferred by Bordetella pertussis Outer Membrane Vesicle Vaccines | |
Jolanda Rigters1  LisaM. Verhagen1  TimH. E. Bindels1  RenéH. M. Raeven1  Elly van Riet1  MerijnL. M. Salverda1  Sabine Kruijer1  JoostP. Uittenbogaard1  Larissa van der Maas1  Naomi van Vlies1  Bernard Metz1  ArnoA. J. van der Ark1  | |
[1] Intravacc (Institute for Translational Vaccinology), Antonie van Leeuwenhoeklaan 9, 3721 MA Bilthoven, The Netherlands; | |
关键词: Bordetella pertussis; virulence factors; pertussis vaccine; OMV; outer membrane vesicles; bvg; | |
DOI : 10.3390/vaccines8030429 | |
来源: DOAJ |
【 摘 要 】
The limited protective immunity induced by acellular pertussis vaccines demands development of novel vaccines that induce broader and longer-lived immunity. In this study, we investigated the protective capacity of outer membrane vesicle pertussis vaccines (omvPV) with different antigenic composition in mice to gain insight into which antigens contribute to protection. We showed that total depletion of virulence factors (bvg(-) mode) in omvPV led to diminished protection despite the presence of high antibody levels. Antibody profiling revealed overlap in humoral responses induced by vaccines in bvg(-) and bvg(+) mode, but the potentially protective responses in the bvg(+) vaccine were mainly directed against virulence-associated outer membrane proteins (virOMPs) such as BrkA and Vag8. However, deletion of either BrkA or Vag8 in our outer membrane vesicle vaccines did not affect the level of protection. In addition, the vaccine-induced immunity profile, which encompasses broad antibody and mixed T-helper 1, 2 and 17 responses, was not changed. We conclude that the presence of multiple virOMPs in omvPV is crucial for protection against Bordetella pertussis. This protective immunity does not depend on individual proteins, as their absence or low abundance can be compensated for by other virOMPs.
【 授权许可】
Unknown